Species Reactivity
Human
Specificity
Detects human Nanog in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse Nanog is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Nanog
Trp153-Val305
Accession # ABZ92376Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin
Applications
Recommended
ConcentrationSample
Intracellular Staining by Flow Cytometry
10 µL/10 6 cells
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry | Detection of Nanog in BG01V Human Cells by Flow Cytometry. Three-day Endoderm-differentiated BG01V human embryonic stem cells (upper image) or untreated BG01V human embryonic stem cells (lower image) were stained with Goat Anti-Human Nanog PE‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC1997P, filled histogram) or isotype control antibody (Catalog # , open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # ) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # ). View our protocol for . |
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: Nanog
Nanog is a member of the homeobox family of DNA binding transcription factors that has been shown to maintain pluripotency of embryonic stem cells. Its expression is high in undifferentiated embryonic stem cells and is down-regulated during embryonic stem cell differentiation, concomitant with loss of pluripotency (1‑3).
References:
Mitsui, K. et al. (2003) Cell 11:631.
Chambers, I. et al. (2003) Cell 113:643.
Hart, A.H. et al. (2004) Dev. Dyn. 230:187.
Long Name:
Nanog Homeobox
Entrez Gene IDs:
79923 (Human); 71950 (Mouse)
Alternate Names:
FLJ12581; FLJ40451; hNanog; homeobox protein NANOG; Homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; Nanog homeobox; Nanog
