Species Reactivity
Mouse
Specificity
Detects mouse MDL-1/CLEC5A. In ELISAs, approximately 50% cross-reactivity with recombinant human MDL‑1 is observed.
Source
Monoclonal Rat IgG2a Clone # 226402
Immunogen
BaF3 mouse pro-B cell line transfected with mouse MDL-1/CLEC5A
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 594
Applications
Recommended
ConcentrationSample
Flow Cytometry
0.25-1 µg/10 6 cells
RAW 264.7 mouse monocyte/macrophage cell line
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: MDL-1/CLEC5A
MDL-1 is an approximately 40 kDa transmembrane glycoprotein belonging to the C-type lectin superfamily (CLEC5A). MDL-1 is expressed on immature myeloid cells, monocytes, macrophages, dendritic cells, neutrophils, NK cells, and osteocytes. It contains a charged lysine in the transmembrane region that enables it to associate with DAP12 and deliver an activating signal. MDL-1 mediates inflammatory responses during autoimmune arthritis and upon binding to Dengue and Japanese encephalitis viruses. The extracellular domain (ECD) of MDL-1 contains a juxtamembrane stalk region and one C-type lectin domain. Within the ECD, mouse and human MDL-1 share 67% amino acid sequence identity. In mouse, alternative splicing generates a short isoform with a 25 aa deletion in the stalk region.
Long Name:
Myeloid DAP12-associating Lectin
Entrez Gene IDs:
23601 (Human); 23845 (Mouse)
Alternate Names:
CLEC5A; CLECSF5; CLECSF5carbohydrate-recognition domain) lectin, superfamilymember 5; C-type lectin domain family 5, member A; C-type lectin superfamily member 5; MDL1; MDL-1; MDL-1MGC138304; Myeloid DAP12-associating lectin 1; myeloid DAP12-associating lectin-1
