Species Reactivity
Mouse
Specificity
Detects mouse Rae‑1 alpha / beta /δ/ epsilon. When tested in flow cytometry against a panel of transfectants expressing different Rae-1 proteins, this antibody strongly recognizes Rae-1 beta and Rae-1δ. It shows weaker staining of Rae-1 alpha and Rae-1 epsilon transfectants. It does not recognize Rae-1 gamma.
Source
Monoclonal Rat IgG2a Clone # 199205
Immunogen
BaF3 mouse pro-B cell line transfected with Rae-1 beta (Accession # NP_033043) and Mouse myeloma cell line NS0-derived recombinant mouse Rae-1δ Leu29-Ser227 (Accession # Q9JI58)
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 594
Applications
Recommended
ConcentrationSample
Flow Cytometry
0.25-1 µg/10 6 cells
RAW 264.7 mouse monocyte/macrophage cell line
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Rae-1
Rae-1 alpha, beta, gamma, δ and epsilon (retinoic acid early transcript 1) comprise a family of closely related (88‑95% amino acid identity) GPI-linked cell surface proteins that function as ligands for mouse NKG2D, an activating receptor expressed on NK and T cells. Rae-1 transcripts are expressed in mouse embryos and several tumor cell lines but are absent from most normal adult tissues. Rae-1 protein expression on tumor cell lines has been implicated in in vivo tumor rejection.
Long Name:
Retinoic Acid Early Transcript 1
Entrez Gene IDs:
8480 (Human); 19368 (Mouse)
Alternate Names:
Rae1; Rae-1
