Species Reactivity
Mouse
Specificity
Detects mouse Rae‑1 alpha / beta / gamma. When tested in flow cytometry against a panel of transfectants expressing different Rae-1 proteins, this antibody recognizes mouse Rae-1 alpha, beta and gamma. It does not recognize Rae-1δ or epsilon.
Source
Monoclonal Rat IgG2a Clone # 199215
Immunogen
BaF3 mouse pro-B cell line transfected with Rae-1 beta and Mouse myeloma cell line NS0-derived recombinant mouse Rae-1δ
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647
Applications
Recommended
ConcentrationSample
Flow Cytometry
0.25-1 µg/10 6 cells
RAW 264.7 mouse monocyte/macrophage cell line
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Rae-1
The Rae-1 family is a group of closely related GPI-linked proteins that function as ligands for NKG2D. Binding of Rae-1 proteins to NKG2D results in the activation of cytolytic activity and/or cytokine production by effector T cells and NK cells.
Long Name:
Retinoic Acid Early Transcript 1
Entrez Gene IDs:
8480 (Human); 19368 (Mouse)
Alternate Names:
Rae1; Rae-1
