Species Reactivity
Human
Specificity
Detects human VEGF in flow cytometry.
Source
Monoclonal Mouse IgG 2A Clone # 23410
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human VEGF
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488
Applications
Recommended
ConcentrationSample
Intracellular Staining by Flow Cytometry
5 µL/10 6 cells
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry | Detection of VEGF in U937 Human Cell Line by Flow Cytometry. U937 human histiocytic lymphoma cell line either treated with 50 ng/mL PMA and 200 ng/mL Calcium Ionomycin for 16 hours (filled histogram) or untreated (open histogram) was stained with Mouse Anti-Human VEGF Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC2931G). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # ) and permeabilized with methanol. View our protocol for . |
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: VEGF
Vascular Endothelial Growth Factor (VEGF) is a soluble protein secreted by a wide variety of cell types. It binds to the receptor tyrosine kinases VEGF R1 (Flt-1) and VEGF R2 (Flk-1). VEGF stimulates vascular endothelial cell proliferation and is a potent inducer of angiogenesis. Several VEGF isoforms occur resulting from alternative mRNA splicing.
Long Name:
Vascular Endothelial Growth Factor
Entrez Gene IDs:
7422 (Human); 22339 (Mouse); 83785 (Rat); 281572 (Bovine); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish)
Alternate Names:
MVCD1; VAS; vascular endothelial growth factor A; Vascular permeability factor; Vasculotropin; VEGF; VEGFA; VEGF-A; VEGFMGC70609; VPF; VPFvascular endothelial growth factor
