Species Reactivity
Human, Mouse
Specificity
Detects human and mouse SOX2 in Western blots.
Source
Monoclonal Mouse IgG 2A Clone # 245610
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human SOX2
Gly135-Met317
Accession # P48431Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488
Applications
Recommended
ConcentrationSample
Intracellular Staining by Flow Cytometry
5 µL/10 6 cells
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry | Detection of SOX2 in NTera‑2 Human Cell Line by Flow Cytometry. NTera‑2 human testicular embryonic carcinoma cell line was stained with Mouse Anti-Human/Mouse SOX2 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC2018G, filled histogram) or isotype control antibody (Catalog # , open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # ) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # ). View our protocol for . |
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: SOX2
SOX2 belongs to the SOX (SRY-like HMG box) family of transcription factors with diverse roles in development. SOX2 functions in specifying the first three lineages present at implantation and in regulating proliferation and differentiation in the developing peripheral nervous system (1-5).
References:
Graham, V. et al. (2003) Neuron 39:749.
Avilion, A.A. et al. (2003) Genes Dev. 17:126.
Kishi, M. et al. (2000) Development 127:791.
Yuan, H. et al. (1995) Genes Dev. 9:2635.
Uwanogho, D. et al. (1995) Mech. Dev. 49:23.
Stevanovic, M. (2003) Mol. Biol. Rep. 30:127.
Long Name:
Transcription Factor SOX2
Entrez Gene IDs:
6657 (Human)
Alternate Names:
ANOP3; MCOPS3; MGC2413; SOX2; SRY (sex determining region Y)-box 2; SRY-related HMG-box gene 2; transcription factor SOX2; transcription factor SOX-2
