Species Reactivity
Human
Specificity
Detects human A33 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse A33 is observed.
Source
Monoclonal Rat IgG 2A Clone # 402104
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human A33
Ile22-Val235
Accession # Q99795Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488
Applications
Recommended
ConcentrationSample
Flow Cytometry
5 µL/10 6 cells
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Flow Cytometry | Detection of A33 in HT‑29 Human Cell Line by Flow Cytometry. HT‑29 human colon adenocarcinoma cell line was stained with Rat Anti-Human A33 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB3080G, filled histogram) or isotype control antibody (Catalog # , open histogram). View our protocol for . |
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: A33
Human A33, also known as GPA33, is a 43 kDa type I transmembrane glycoprotein that belongs to the CTX (Cortical Thymocyte Marker in Xenopus) family of cell adhesion molecules within the immunoglobulin superfamily. Other family members include CXADR, ESAM, BT-IgSF, CD2 and JAM-A-C. The extracellular domain (ECD) of human A33 is 214 amino acids (aa) in length and contains one V-type and one C2-type Ig-like domain. This ECD is 80%, 74% and 71% aa identical to canine, bovine and mouse A33 ECD, respectively. A33 is likely to be involved in cell-cell adhesion between epithelial cells.
Long Name:
Cell Surface A33 Antigen
Entrez Gene IDs:
10223 (Human); 59290 (Mouse)
Alternate Names:
A33; cell surface A33 antigen; glycoprotein A33 (transmembrane); Glycoprotein A33; GPA33; MGC129986; MGC129987; transmembrane glycoprotein A33
