Human IL-13 R alpha 1 Alexa Fluor 700 MAb (Clone 419718) 100 TESTS

• Species Reactivity

  Human

• Specificity

  Detects human IL-13 R alpha 1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse IL-13 R alpha 1, recombinant human (rh) IL-13 R alpha 2, rhIL-4 R, rhIL-5 R beta, or rhIL-9 R is observed.

• Source

  Monoclonal Mouse IgG    _2B Clone # 419718

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human IL‑13 R alpha 1    
  Ala27-Thr343    
  Accession # P78552

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 700

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  5 µL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-13 R alpha 1

Two type 1 membrane proteins belonging to the hemopoietin receptor family have been cloned and shown to bind IL-13 with differing affinities. The lower affinity IL-13 binding protein, previously designated IL-13 R alpha, or NR4, is now referred to as IL-13 R alpha 1. The high-affinity IL-13 binding protein, previously also designated IL-13 R or IL-13 R alpha ', is now referred to as IL-13 R alpha 2.

The human IL-13 R alpha 1 was originally cloned based on sequence homology to the mouse IL-13 R alpha 1. The IL-13 R alpha 1 cDNA encodes a 427 amino acid (aa) precursor protein with a putative 21 aa signal peptide, a 324 aa extracellular domain, a 23 aa transmembrane region and a 59 aa cytoplasmic tail. Human and mouse IL-13 R alpha 1 share 76% aa sequence identity. The extracellular domain of IL-13 R alpha 1 is also closely related to that of IL-13 R alpha 2. IL‑13 R alpha 1 has been shown to combine with the IL‑4 R alpha to form a high-affinity receptor complex capable of transducing an IL-13-dependent proliferative signal. The role of IL-13 R alpha 2 in IL-13 signaling remains to be elucidated.

• References:

1. Caput, D. et al. (1996) J. Biol. Chem. 271:16921.

2. Donaldson, D.D. et al. (1998) J. Immunol. 161:2317.

3. Aman, M.J. et al. (1996) J. Biol. Chem. 271:29265.

4. Hilton, D.J. et al. (1996) Proc. Natl. Acad. Sci. USA 93:497.

5. Zhang, J.G. et al. (1997) J. Biol. Chem. 272:9474.

• Long Name:

  Interleukin 13 Receptor alpha 1

• Entrez Gene IDs:

  3597 (Human); 16164 (Mouse)

• Alternate Names:

  bB128O4.2.1 (interleukin 13 receptor, alpha 1); Cancer/testis antigen 19; CD213a1 antigen; CD213a1; CT19; IL-13 R alpha 1; IL13 receptor alpha-1 chain; IL-13 receptor subunit alpha-1; IL13R alpha 1; IL-13R subunit alpha-1; IL13R; IL13RA; IL-13Ra; IL13RA1; IL-13Ra1; IL-13R-alpha-1; interleukin 13 receptor, alpha 1; interleukin-13 receptor subunit alpha-1; NR4