Species Reactivity
Human
Specificity
Detects human CLEC9a in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) CLEC1, 2, 2A, 3B, 10A, 12B, 14A, rhCD302/CLEC13a, rhMICL, or recombinant mouse CLEC9a is observed.
Source
Monoclonal Mouse IgG 1 Clone # 683409
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CLEC9a
Lys57-Val241
Accession # Q6UXN8Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488
Applications
Recommended
ConcentrationSample
Flow Cytometry
5 µL/10 6 cells
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Flow Cytometry | Detection of CLEC9a in Human Peripheral Blood Cells by Flow Cytometry. Human peripheral blood cells gated on CD3-CD141+ cells were stained with Mouse Anti-Human HLA‑DR PerCP‑conjugated Monoclonal Antibody (Catalog # ) and either (A) Mouse Anti-Human CLEC9a Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB6049G) or (B) Mouse IgG1 Alexa Fluor 488 Isotype Control (Catalog # ). View our protocol for . |
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: CLEC9a
CLEC9a (C-type lectin domain family 9 member A), also known as DNGR-1, is a type II transmembrane glycoprotein member of the C‑type lectin superfamily (1, 2). Mature human CLEC9a consists of a 35 amino acid (aa) cytoplasmic domain with an ITAM-like motif, a 21 aa transmembrane segment, and a 185 extracellular domain (ECD) that contains a stalk region and one C-type lectin domain (CTLD) (3‑5). Within the ECD, human CLEC9a shares 57% aa sequence identity with mouse and rat CLEC9a. Although the CTLD of CLEC9a structurally resembles that of other C-type lectins, it lacks the conserved residues that typically mediate calcium and carbohydrate binding. CLEC9a is expressed as a disulfide-linked homodimer of approximately 50 kDa N-glycosylated subunits (3, 5). Human CLEC9a expression is restricted to a subpopulation of BDCA-3+ conventional dendritic cells (cDC) and CD16- monocytes (3‑5). BDCA-3+ cDC are analagous to mouse CD8+ cDC which are specialized in antigenic cross-presentation in complex with MHC class I molecules (6). In mouse, CLEC9a is additionally expressed on plasmacytoid dendritic cells (4, 5). CLEC9a ligation enhances antigen uptake and processing, leading to presentation on MHC class I and cytotoxic T cell (CTL) priming (3‑5). In mouse, CLEC9a recognizes normally intracellular determinant(s) of necrotic cells and mediates their uptake by the dendritic cell (7). The subsequent antigenic cross-presentation to CTL is important for clearing necrotic cellular debris (7). CLEC9a signaling triggers activation of the tyrosine kinase Syk (3, 7).
References:
Huysamen, C. and G.D. Brown (2009) FEMS Microbiol. Lett. 290:121.
Geijtenbeek, T.B.H. et al. (2004) Annu. Rev. Immunol. 22:33.
Huysamen, C. et al. (2008) J. Biol. Chem. 283:16693.
Caminschi, I. et al. (2008) Blood 112:3264.
Sancho, D. et al. (2008) J. Clin. Invest. 118:2098.
Dudziak, D. et al. (2007) Science 315:107.
Sancho, D. et al. (2009) Nature 458:899.
Long Name:
C-type Lectin-like Receptor 9
Entrez Gene IDs:
283420 (Human); 232414 (Mouse)
Alternate Names:
CD370; CLEC9a; C-type lectin domain family 9 member A; C-type lectin domain family 9, member A; DNGR1; HEEE9341; UNQ9341
