Species Reactivity
Mouse
Specificity
Detects mouse PD-1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human PD-1 is observed.
Source
Monoclonal Rat IgG 2A Clone # 766104
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse PD-1
Leu25-Gln167
Accession # Q02242Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488
Applications
Recommended
ConcentrationSample
Flow Cytometry
5 µL/10 6 cells
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Flow Cytometry | Detection of PD‑1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes either (A) resting or (B) treated with 5 μg/mLof PHA for 72 hours were stained with Rat Anti-Mouse PD‑1 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB7738G, filled histogram) or isotype control antibody (Catalog # , open histogram). View our protocol for . |
Flow Cytometry | Detection of PD‑1 in Mouse Thymocytes by Flow Cytometry. Mouse thymocytes gated on CD3+ CD8+ cells were stained with Rat Anti-Mouse CD4 Alexa Fluor® 405‑conjugated Monoclonal Antibody (Catalog # ) and either (A) Rat Anti-Mouse PD‑1 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB7738G) or (B) Rat IgG2A Alexa Fluor 488 Isotype Control (Catalog # ). View our protocol for . |
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: PD-1
Programmed Death-1 (PD-1) is a type I transmembrane protein belonging to the CD28/CTLA-4 family of immunoreceptors that mediate signals for regulating immune responses (1). Other members of this family include CD28, CTLA-4, and ICOS (2-4). PD-1 is most closely related to CTLA-4 and shares approximately 24% amino acid (aa) sequence identity. The mouse PD-1 gene encodes a 288 aa protein with a putative 20 aa signal peptide, a 149 aa extracellular region with one immunoglobulin-like V-type domain, a 21 aa transmembrane domain, and a 98 aa cytoplasmic region. The cytoplasmic tail contains two tyrosine residues that form the immunoreceptor tyrosine-based inhibitory motif (ITIM) and immunoreceptor tyrosine-based switch motif (ITSM) that are important in mediating PD-1 signaling. Mouse and human PD-1 share approximately 69% aa sequence identity. Two B7 family proteins, PD-L1 (also called B7-H1) and PD-L2, have been identified as PD-1 ligands (5, 6). PD-1 is expressed on activated T cells, B cells, myeloid cells, and on a subset of thymocytes. PD-1 deficient mice have a defect in peripheral tolerance and spontaneously develop autoimmune diseases. Binding of PD-1 to PD-L1 or PD-L2 results in the inhibition of TCR-mediated proliferation and cytokine production as well as BCR-mediated signaling. PD-1 likely has an inhibitory role in regulating immune responses (1-4).
References:
Ishida, Y. et al. (1992) EMBO J. 11:3887.
Sharpe, A.H. and G.J. Freeman (2002) Nat. Rev. Immunol. 2:116.
Coyle, A. and J. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
Nishimura, H. and T. Honjo (2001) Trends Immunol. 22:265.
Latchman Y. et al. (2001) Nat. Immunol. 2:261.
Tamura, H. et al. (2001) Blood 97:1809.
Long Name:
Programmed Death-1
Entrez Gene IDs:
5133 (Human); 18566 (Mouse); 301626 (Rat); 486213 (Canine); 102123659 (Cynomolgus Monkey)
Alternate Names:
CD279 antigen; CD279; hPD-1; PD-1; PD1hPD-l; PDCD1; programmed cell death 1; programmed cell death protein 1; Protein PD-1; SLEB2
