Species Reactivity
Mouse
Specificity
Detects mouse Galectin-9 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Galectin-9 or recombinant mouse Galectin-4 is observed.
Source
Monoclonal Rat IgG 2B Clone # 766428
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse Galectin-9 (short isoform)
Ala2-Thr322
Accession # O08573-2Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488
Applications
Recommended
ConcentrationSample
Intracellular Staining by Flow Cytometry
5 µL/10 6 cells
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry | Detection of Galectin‑9 in Mouse Thymocytes by Flow Cytometry. Mouse thymocytes were stained with Rat Anti-Mouse Galectin‑9 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC3535G, filled histogram) or isotype control antibody (Catalog # , open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # ) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # ). View our protocol for . |
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: Galectin-9
Mouse Galectin-9 ( beta -Galactoside-binding Lectin 9) is a 36-39 kDa, secreted, S-type lectin. It is 353 amino acids (aa) in length and contains no identifiable signal sequence. There are two distinct carbohydrate-binding regions (aa 81-87 and 285-291) that are joined by a linker region (aa 148-205). At least one alternate splice form exists that shows a 31 aa insertion between aa 147-148. The short form is expressed on fibroblasts, hepatocytes, endothelial cells and astrocytes. The long form is expressed on intestinal epithelium. Galectin-9 binds TIM-3 on Th1 cells, inducing apoptosis. Mouse Galectin-9 is 69% and 85% aa identical to human and rat Galectin-9, respectively.
Entrez Gene IDs:
3965 (Human); 16859 (Mouse)
Alternate Names:
Ecalectin; GAL9; gal-9; galectin 9; Galectin9; Galectin-9; HUAT; lectin, galactoside-binding, soluble, 9; LGALS9; LGALS9A; MGC117375; MGC125973; MGC125974; Tumor antigen HOM-HD-21; urate transporter/channel protein
