Species Reactivity
Human
Specificity
Detects human Siglec-8 in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Siglec-7, -9, or -14 is observed.
Source
Monoclonal Mouse IgG 1 Clone # 837535
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Siglec-8
Met17-Ala363
Accession # Q9NYZ4Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin
Applications
Recommended
ConcentrationSample
Flow Cytometry
10 µL/10 6 cells
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Flow Cytometry | Detection of Siglec-8 in Human Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Rat Anti-Human CCR3 APC‑conjugated Monoclonal Antibody (Catalog # ) and either (A) Mouse Anti-Human Siglec-8 PE‑conjugated Monoclonal Antibody (Catalog # FAB7975P) or (B) Mouse IgG1 Phycoerythrin Isotype Control (Catalog # ). View our protocol for . |
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: Siglec-8
Siglec-8 (Sialic acid-binding Ig-like lectin 8), formerly called SAF-2 (sialoadhesin family member 2) and designated CD329, is a 75 kDa member of the inhibitory CD33‑related SIGLEC family within the Ig superfamily of proteins. An alternately spliced 48 kDa short form lacks most of the intracellular domain, while another isoform lacks the second of three extracellular Ig domains, representing amino acids (aa) 152-245 of the 499 aa long form. Siglec-8 is expressed exclusively on eosinophils, mast cells, and weakly on basophils, and is a marker for eosinophil terminal differentiation. It specifically recognizes 6’ sulfated sialyl Lewis X carbohydrate structures. Engagement results in eosinophils apoptosis, or mast cell inhibition of degranulation. Eosinophil Siglec F is the mouse paralog of human Siglec-8, but shares<40% amino acid sequence identity.
Long Name:
Sialic Acid Binding Ig-like Lectin 8
Entrez Gene IDs:
27181 (Human)
Alternate Names:
CD329 antigen; CDw329; MGC59785; SAF2; SAF2SAF-2; sialic acid binding Ig-like lectin 8; Sialoadhesin family member 2; Siglec8; Siglec-8; SIGLEC8L; SIGLEC8Lsialic acid-binding Ig-like lectin 8
