Human IL-10 R beta Alexa Fluor 488 MAb (Clone 90220) 100 TESTS

• Species Reactivity

  Human

• Specificity

  Detects human IL-10 R beta  in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL‑10 R alpha, rhIFN-gamma  RI, and rhIFN-gamma  R2 is observed.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 90220

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human IL-10 R beta    
  Met20-Ser220    
  Accession # Q08334

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 488

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  5 µL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-10 R beta

IL-10 mediates its biological activities via binding to a heteromeric receptor complex consisting of two distinct type II cytokine receptor subunits, the ligand binding IL-10 R alpha and the IL-10 R beta which does not bind IL-10 by itself but is required for signal transduction. The cDNA for human IL-10 R beta encodes a 325 amino acid (aa) type I transmembrane precursor protein with a 20 aa signal sequence, a 200 aa extracellular region, a 29 aa transmembrane segment and a 76 aa cytoplasmic domain. Within the extracellular region, there are two 100 aa subdomains that resemble the constant region of immunoglobulins. This structural motif is responsible for the alternative designation of IL-10 R beta as CRF2-4 (the 4^th member of the cytokine receptor family class II/2). Human and mouse IL-10 R beta share approximately 69% aa sequence identity. Binding of the non-covalent IL-10 dimer to two IL-10 R alpha chains recruits two IL-10 R beta chains resulting in the activation and phosphorylation of the signaling cascade involving JAK1, TYK2, and STAT3. IL-10 R beta  is expressed ubiquitously. IL-10 R beta is also a component of the IL-22 receptor complex consisting of the IL-10 R beta chain and IL-22 R, another type II cytokine receptor family member.

• References:

1. Donnelly, R.P. et al. (2004) J. Leukoc. Biol. 76:314.

2. Donnelly, R.P. et al. (1999) J. Interferon Cytokine Res. 19:563.

3. Kotenko, S.V. et al. (2000) J. Biol. Chem. 276:2725.

4. Liu, Y. et al. (1994) J. Immunol. 152:1821.

5. Lutfalla, G. et al. (1993) Genomics 16:366.

6. Kotenko, S.V. et al. (1997) EMBO J. 16:5894.

• Long Name:

  Interleukin 10 Receptor beta

• Entrez Gene IDs:

  3588 (Human); 16155 (Mouse)

• Alternate Names:

  CDw210b antigen; CDw210b; CRF2-4IL-10R subunit 2; CRFB4; CRFB4human transmembrane receptor protein; cytokine receptor10CDW210B; D21S58; D21S66; IBD25; IL-10 R beta; IL-10 receptor subunit beta; IL10R beta; IL-10R2; IL-10R2IL-10R subunit beta; IL10RB; IL-10Rb; interleukin 10 receptor, beta; Interleukin-10 receptor subunit 2; interleukin-10 receptor subunit beta; member 4