Species Reactivity
Mouse
Specificity
Detects mouse B220/CD45R in flow cytometry.
Source
Monoclonal Rat IgG 2A Clone # RA3-6B2
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Abelson mouse leukemia virus-induced pre-B tumor cells
Formulation
Supplied in a saline solution containing BSA and Sodium Azide. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Alexa Fluor 405
Applications
Recommended
ConcentrationSample
Flow Cytometry
5 µL/10 6 cells
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Flow Cytometry | Detection of B220/CD45R in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC‑conjugated Monoclonal Antibody (Catalog # ) and either (A) Rat Anti-Mouse B220/CD45R Alexa Fluor® 405‑conjugated Monoclonal Antibody (Catalog # FAB1217V) or (B) Rat IgG2A Alexa Fluor 405 Isotype Control (Catalog # ). View our protocol for . |
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: B220/CD45R
B220 is the mouse CD45 isoform CD45R, which is predominantly expressed on all B lymphocytes, including pro-, mature and activated B cells. The level of B220 antigen expression on cells of the B cell lineage is developmentally regulated, and the antibody described here is commonly used to identify this B cell marker. RA3-6B2 has been reported to inhibit in vivo B cell responses. The extracellular region of mouse B220 shares 41% amino acid sequence identity with the extracellular region of human CD45.
Long Name:
Cluster of Differentiation 45
Entrez Gene IDs:
5788 (Human); 19264 (Mouse); 24699 (Rat)
Alternate Names:
B220; CD45 antigen; CD45R; EC 3.1.3.48; L-CA; LY5; protein tyrosine phosphatase, receptor type, C; receptor-type tyrosine-protein phosphatase C; T200 glycoprotein; T200 leukocyte common antigen; T200receptor type, c polypeptide
