Species Reactivity
Human
Specificity
Detects human TRA‑1‑85 antigen in flow cytometry.
Source
Monoclonal Mouse IgG 1 Clone # TRA-1-85
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
2120Ep human embryonal carcinoma cell line
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 700
Applications
Recommended
ConcentrationSample
Flow Cytometry
5 µL/10 6 cells
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Flow Cytometry | Detection of TRA‑1‑85/CD147 in Human PBMCs by Flow Cytometry. Human peripheral blood lymphocytes and monocytes were stained with either (A) Mouse Anti-Human TRA‑1‑85/CD147 Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB3195N) or (B) Mouse IgG1 Alexa Fluor 700 Isotype Control (Catalog # ). View our protocol for . |
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: TRA-1-85/CD147
The TRA-1-85 antigen, also known as OKa blood group antigen, is a specific epitope within the protein known as Basigin, EMMPRIN and CD147. It is a cell surface determinant expressed on almost all human cell types. This antibody has been used in somatic cell hybrid studies to identify tissues of partial human origin (1, 2).
References:
Williams, B.P. et al. (1988) Immunogenetics. 27:322.
Spring, F.A. et al. (1997) Eur. J. Immunol. 27:891.
Entrez Gene IDs:
682 (Human)
Alternate Names:
OKa Blood Group Antigen; TRA185; TRA-1-85
