Mouse PDGF R alpha Phycoerythrin Affinity Purified PAb 100 TESTS

• Species Reactivity

  Mouse

• Specificity

  Detects mouse PDGF R alpha in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) PDGF R alpha, rhPDGF R beta, and recombinant mouse PDGF R beta is observed.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse PDGF R alpha    
  Leu25-Glu524 (Asp65Glu, Gly439Ala, Thr440Ala)    
  Accession # P26618

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Phycoerythrin

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  10 µL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: PDGF R alpha

The platelet-derived growth factor (PDGF) family consists of proteins derived from four genes (PDGF-A, -B, -C, and -D) that form disulfide-linked homodimers (PDGF-AA, -BB, -CC, and -DD) and a heterodimer (PDGF-AB) (1, 2). These proteins regulate diverse cellular functions by binding to and inducing the homo- or hetero-dimerization of two receptors (PDGF R alpha  and R beta ). Whereas alpha / alpha homo-dimerization is induced by PDGF-AA, -BB, -CC, and -AB, alpha / beta  hetero-dimerization is induced by PDGF-AB, -BB, -CC, and -DD, and beta / beta  homo-dimerization is induced only by PDGF-BB and -DD (1-4). Both PDGF R alpha  and R beta are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF, and Flt-3 ligand. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. Ligand-induced receptor dimerization results in autophosphorylation in trans resulting in the activation of several intracellular signaling pathways that can lead to cell proliferation, cell survival, cytoskeletal rearrangement, and cell migration. Many cell types, including fibroblasts and smooth muscle cells, express both the alpha  and beta  receptors. Others have only the alpha  receptors (oligodendrocyte progenitor cells, mesothelial cells, liver sinusoidal endothelial cells, astrocytes, platelets, and megakaryocytes) or only the  beta receptors (myoblasts, capillary endothelial cells, pericytes, T cells, myeloid hematopoietic cells, and macrophages) (1, 2). Recombinant mouse and human soluble PDGF R beta  bind PDGF with high affinity and are potent PDGF antagonists.

• References:

1. Betsholtz, C. et al. (2001) BioEssays 23:494.

2. Ostman, A. and A.H. Heldin (2001) Advances in Cancer Research 80:1.

3. Gilbertson, D. et al. (2001) J. Biol. Chem. 276:27406.

4. LaRochells, W.J. et al. (2001) Nature Cell Biol. 3:517.

• Long Name:

  Platelet-derived Growth Factor Receptor alpha

• Entrez Gene IDs:

  5156 (Human); 18595 (Mouse)

• Alternate Names:

  alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A; CD140a antigen; CD140a; EC 2.7.10; EC 2.7.10.1; MGC74795; PDGF R alpha; PDGFR2; PDGFRA; PDGFRA/BCR fusion; PDGF-R-alpha; platelet-derived growth factor receptor, alpha polypeptide; rearranged-in-hypereosinophilia-platelet derived growth factor receptor alphafusion protein; RHEPDGFRA