Human BCMA/TNFRSF17 Alexa Fluor 700 Affinity Purified PAb 100 TESTS

• Species Reactivity

  Human

• Specificity

  Detects human BCMA in ELISAs and Western blots. In sandwich ELISAs, less than 0.1% cross-reactivity with recombinant human (rh) APRIL, rhBAFF, rhTACI, and recombinant mouse BCMA is observed.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human BCMA/TNFRSF17    
  Met1-Ala54    
  Accession # Q6PE46

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 700

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  5 µL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: BCMA/TNFRSF17

BCMA, B cell maturation, is a member of the TNF receptor superfamily. It has been designated TNFRSF17. BCMA is a type III membrane protein containing one extracellular cysteine rich domain. Within the TNFRSF, it shares the highest homology with TACI. BCMA and TACI have both been shown to bind to APRIL and BAFF, members of the TNF ligand superfamily. BCMA expression has been found in immune organs and mature B cell lines. Although some expression has been observed at the cell surface, BCMA appears to be localized to the Golgi compartment. The binding of BCMA to APRIL or BAFF has been shown to stimulate IgM production in peripheral blood B cells and increase the survival of cultured B cells. This data suggests that BCMA may play an important role in B cell development, function and regulation. Human BCMA is a 184 amino acid (aa) protein consisting of a 54 aa extracellular domain, a 23 aa transmembrane domain, and a 107 aa intracellular domain. Mouse and human BCMA share 62% amino acid identity.

• References:

1. Madry, C. et al. (1998) Int. Immunol. 10:1693.

2. Gras, M. et al. (1995) Int. Immunol. 7:1093.

3. Kwon, B. et al. (1999) Curr. Opin. Immunol. 11:340.

4. Marsters, S. et al. (2000) Curr. Biol. 10:785.

5. Thompson, J. et al. (2000) J. Exp. Med. 192:129.

• Long Name:

  B Cell Maturation Factor

• Entrez Gene IDs:

  608 (Human); 21935 (Mouse); 287034 (Rat); 102145399 (Cynomolgus Monkey)

• Alternate Names:

  B cell maturation antigen; B-cell maturation protein; BCMA; BCMAtumor necrosis factor receptor superfamily member 17; BCMB-cell maturation factor; CD269 antigen; CD269; TNFRSF17; tumor necrosis factor receptor superfamily, member 17