Species Reactivity
Human
Specificity
Detects human Integrin alpha 1/CD49a in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) Integrin alpha 2, rhIntegrin alpha 11, recombinant mouse (rm) Integrin alpha 2, and rmIntegrin alpha 11 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Integrin alpha 1/CD49a
Phe29-Pro1141
Accession # P56199Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin
Applications
Recommended
ConcentrationSample
Flow Cytometry
10 µL/10 6 cells
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Flow Cytometry | Detection of Integrin alpha 1/CD49a in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Sheep Anti-Human Integrin alpha 1/CD49a APC‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB5676A, filled histogram) or isotype control antibody (Catalog # , open histogram). View our protocol for . |
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: Integrin alpha 1/CD49a
Integrin alpha 1 (also VLA-1, CD49a and Laminin and Collagen Receptor) is a 190‑210 kDa member of the integrin alpha chain family of molecules. It is found on smooth muscle cells, osteoblasts, adipocytes and intestinal epithelium. Integrin alpha 1 forms a noncovalent heterodimer with Integrin beta 1, and serves as a divalent-cation dependent receptor for collagen types I, IV, VI, XIII and XVI. It also binds the diarrhea-associated NSP4 enterotoxin of rotavirus. Mature human Integrin alpha 1 is a 1151 amino acid (aa) type I transmembrane glycoprotein that contains a 1113 aa extracellular domain (ECD) and a 15 aa cytoplasmic tail. The ECD contains one vWFA/I‑domain (aa 147‑360) that binds collagen, plus multiple divalent cation binding sites. Potential splice variants exist that show a two and four aa substitution for aa 765‑1179. Over aa 29‑1141 (the ECD), human Integrin alpha 1 shares 88% aa identity with mouse Integrin alpha 1.
Entrez Gene IDs:
3672 (Human); 109700 (Mouse); 25118 (Rat)
Alternate Names:
CD49 antigen-like family member A; CD49a antigen; CD49a; Integrin alpha 1; integrin, alpha 1; ITGA1; Laminin and collagen receptor; very late activation protein 1; VLA-1; VLA1integrin alpha-1
