Species Reactivity
Mouse
Specificity
Detects mouse Nanog in Western blots. In this format, approximately 50% cross-reactivity with recombinant human Nanog is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse Nanog
Trp154-Leu262
Accession # Q80Z64Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488
Applications
Recommended
ConcentrationSample
Immunocytochemistry
8-25 µg/mL
See below
Intracellular Staining by Flow Cytometry
5 µL/10 6 cells
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry | Detection of Nanog in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem cell line either untreated (dark orange filled histogram) or treated with retinoic acid for 3 days (light orange filled histogram) was stained with Goat Anti-Mouse Nanog Alexa Fluor® 488‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC2729G) or isotype control antibody (Catalog # , blue open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # ) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # ). View our protocol for . |
Immunocytochemistry | Nanog in D3 Mouse Embryonic Stem Cells. Nanog was detected in immersion fixed D3 mouse embryonic stem cell line on irradiated mouse embryonic fibroblasts using Goat Anti-Mouse Nanog Alexa Fluor® 488‑conjugated Antigen Affinity-purified Polyclonal Antibody (green; Catalog # IC2729G) at 10 µg/mL for 3 hours at room temperature. Cells were counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for . |
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: Nanog
Nanog is a member of the homeobox family of DNA binding transcription factors that has been shown to maintain pluripotency of embryonic stem cells. Its expression is high in undifferentiated embryonic stem cells and is down-regulated during embryonic stem cell differentiation, concomitant with loss of pluripotency (1‑3).
References:
Mitsui, K. et al. (2003) Cell 11:631.
Chambers, I. et al. (2003) Cell 113:643.
Hart, A.H. et al. (2004) Dev. Dyn. 230:187.
Long Name:
Nanog Homeobox
Entrez Gene IDs:
79923 (Human); 71950 (Mouse)
Alternate Names:
FLJ12581; FLJ40451; hNanog; homeobox protein NANOG; Homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; Nanog homeobox; Nanog
