Species Reactivity
Human
Specificity
Detects human Coagulation Factor III/Tissue Factor in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse Coagulation Factor III/Tissue Factor is observed.
Source
Monoclonal Mouse IgG 1 Clone # 323519
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Coagulation Factor III
Gly34-Glu251
Accession # P13726Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488
Applications
Recommended
ConcentrationSample
Flow Cytometry
5 µL/10 6 cells
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Flow Cytometry | Detection of Coagulation Factor III/Tissue Factor in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD14 APC‑conjugated Monoclonal Antibody (Catalog # ) and either (A) Mouse Anti-Human Coagulation Factor III/Tissue Factor Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB23391G) or (B) Mouse IgG1 Alexa Fluor 488 Isotype Control (Catalog # ). View our protocol for . |
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: Coagulation Factor III/Tissue Factor
Coagulation Factor III/Tissue Factor (TF), also known as thromboplastin and CD142, is a 46 kDa integral membrane protein that is part of the extrinsic pathway of coagulation. It functions as a protein cofactor/receptor for Coagulation Factor VII, which is synthesized in the liver and circulates in the plasma (1). Upon binding to TF, the inactive Factor VII is rapidly converted into activated Factor VIIa. The resulting 1:1 complex of Factor VIIa and TF activates the coagulation pathway, and as a soluble form, plays an important coagulation-independent role in processes such as angiognesis (2-4). Synthesized as a 295 amino acid (aa) precursor, TF consists of a signal peptide (aa 1-32) and the mature chain (aa 33-295). The mature region contain an extracellular region (aa 33-251), followed by a transmembrane segment (aa 252-274) and a cytoplasmic tail (aa 275-295) (5-8). TF is found on endothelial cells, monocytes and vascular smooth muscle cells. Over aa 33-251, human and mouse TF share 58% aa sequence identity.
References:
Morrissey, J.H. (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. (ed) San Diego, Academic Press, p. 1659.
Versteeg, H.H. et al. (2003) Carcinogenesis 24:1009.
Gedding, J.E. and N. Mackman (2014) Thromb. Haemost. 111:570.
Hobbs, J.E. et al. (2007) Thromb. Res. 120 Suppl 2:S13.
Scarpati, E.M. et al. (1987) Biochemistry 26:5234.
Fisher, K.L. et al. (1987) Thromb. Res. 48:89.
Morrissey, J.H. et al. (1987) Cell 50:129.
Spicer, E.K. (1987) Proc. Natl. Acad. Sci. USA 84:5148.
Entrez Gene IDs:
2152 (Human); 14066 (Mouse)
Alternate Names:
CD142 antigen; CD142; coagulation factor III (thromboplastin, tissue factor); Coagulation Factor III; F3; FLJ17960; TF; TFA; Thromboplastin; Tissue Factor
