Assay Length
3.5 hours
Specificity
Measures cyclic AMP levels in various samples
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules.Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
Control Available
<div class="add-to-cart" data-type="anc" data-catbase="QC52" data-return="camp-parameter-assay-kit_kge002b" data-produrl="parameter-immunoassay-control-set-764-for-camp_qc52" data-title="Parameter Immunoassay Control Set 764 for cAMP" data-catnums="QC52">
QC52, Parameter Immunoassay Control Set 764 for cAMP -
Product Summary
The Parameter cAMP Immunoassay is a 3.5 hour competitive enzyme immunoassay designed to measure cAMP in cell culture supernates, cell lysates, plasma, serum, saliva, and urine.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 22.6 | 68.7 | 116 | 21.7 | 70.4 | 119 |
| Standard Deviation | 1.37 | 2.55 | 2.54 | 2.2 | 6 | 7.3 |
| CV% | 6 | 3.7 | 2.2 | 10.3 | 8.6 | 6.1 |
Cell Lysates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 18.5 | 56.9 | 96.1 | 17 | 51.8 | 89.2 |
| Standard Deviation | 0.89 | 1.74 | 2.73 | 1.3 | 3.5 | 6.9 |
| CV% | 4.8 | 3.1 | 2.8 | 7.4 | 6.8 | 7.7 |
Recovery
The recovery of cAMP spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 104 | 86-119 |
| Cell Lysis Buffer (n=1) | 91 | 87-94 |
| EDTA Plasma (n=4) | 105 | 92-120 |
| Heparin Plasma (n=4) | 98 | 85-111 |
| Saliva (n=4) | 97 | 80-117 |
| Serum (n=4) | 103 | 97-114 |
| Urine (n=6) | 100 | 91-119 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of cAMP were serially diluted with the appropriate diluent to produce samples with values within the dynamic range of the assay.
Preparation and Storage
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Background: cAMP
Adenosine 3,5-cyclic monophosphate (cAMP) is one of the most important intracellular second messengers. Several hormones are known to activate cAMP and subsequent signaling cascades via their indirect influence on adenylate cyclase, the enzyme responsible for the conversion of ATP to cAMP.
Long Name
Cyclic AMP
Alternate Names
cAMP;
