Format
96-well strip plate
Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Urine (10 uL)
Sensitivity
0.047 ng/mL
Assay Range
0.1 - 25 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Specificity
Corticosterone in human, mouse, and rat samples.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Parameter Corticosterone assay is a 3.5 hour competitive enzyme immunoassay designed to measure Corticosterone in cell culture supernates, serum, plasma, and urine.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of kit components.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 1.24 | 3.98 | 8.05 | 1.39 | 4.08 | 8.21 |
| Standard Deviation | 0.078 | 0.179 | 0.605 | 0.099 | 0.241 | 0.462 |
| CV% | 6.3 | 4.5 | 7.5 | 7.1 | 5.9 | 5.6 |
Recovery
The recovery of Corticosterone spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 114 | 107-124 |
| Human EDTA Plasma (n=4) | 108 | 87-127 |
| Human Heparin Plasma (n=4) | 107 | 97-122 |
| Human Serum (n=4) | 110 | 92-130 |
| Mouse EDTA Plasma (n=4) | 104 | 96-111 |
| Mouse Serum (n=4) | 96 | 79-113 |
| Rat EDTA Plasma (n=4) | 98 | 77-114 |
| Rat Serum (n=4) | 99 | 87-108 |
| Urine (n=4) | 105 | 92-121 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Corticosterone were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Preparation and Storage
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Corticosterone
Corticosterone, also known as Kendall's compound B and Reichstein substance B, is a glucocorticoid secreted by the adrenal gland cortex. It is produced in response to adrenal cortex stimulation by adrenocorticotropic hormone (ACTH) and is the precursor of aldosterone. Stress increases corticosteroid production. Corticosterone level is measured as an indicator of stress in patients with dietary restrictions, long-term memory impairment, and burn injuries.
Alternate Names
Corticosterone;
