Format
96-well strip plate
Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Urine (50 uL)
Sensitivity
0.31 ng/mL
Assay Range
0.3 - 20 ng/mL (Cell Culture Supernates, Serum, Plasma, EDTA Plasma, Heparin Plasma, Urine)
Specificity
Measures TXB 2 levels in various samples
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules.Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
Control Available
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QC179, Parameter Immunoassay Control Set 931 for Thromboxane B2 -
Product Summary
The Parameter TXB 2 Immunoassay is a 3.5 hour competitive enzyme immunoassay designed to measure TXB2 in cell culture supernates, serum, plasma, and urine.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 3.87 | 6.95 | 13.2 | 3.86 | 7.05 | 14.4 |
| Standard Deviation | 0.23 | 0.38 | 0.51 | 0.34 | 0.45 | 0.74 |
| CV% | 5.9 | 5.5 | 3.9 | 8.9 | 6.4 | 5.1 |
Recovery
The recovery of TXB2 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 98 | 88-110 |
| EDTA Plasma (n=4) | 94 | 86-105 |
| Heparin Plasma (n=4) | 97 | 87-107 |
| Serum (n=4) | 98 | 86-111 |
| Urine (n=4) | 98 | 84-111 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of TXB 2 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Preparation and Storage
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Thromboxane B2
Thromboxane A2 (TXA2), is an eicosanoid involved in platelet aggregation, vasoconstriction, and reproductive functions. However, as TXA2 is rapidly degraded in vivo, TXA2 production is typically examined by assaying the more stable Thromboxane B2 (TXB2) or 11-dehydro-TXB2 (11-d-TXB2) derivatives. Levels of these metabolites are helpful markers in the study of diseases including liver cirrhosis, cystic fibrosis, mastocytosis, systemic lupus erythematosus, thrombosis diseases, and others.
Long Name
Thromboxane B2
Alternate Names
Thromboxane B2; TXB2;
