Format
96-well strip plate
Assay Length
2-3 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (150 uL), Cell Lysates (150 uL), Serum (150 uL), EDTA Plasma (150 uL), Heparin Plasma (150 uL), Urine (150 uL)
Sensitivity
0.055 uM
Assay Range
0.0 - 17 uM
Specificity
TBARS in various samples
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Control Available
<div class="add-to-cart" data-type="anc" data-catbase="QC164" data-return="tbars-parameter-assay-kit_kge013" data-produrl="parameter-immunoassay-control-set-752-for-tbars_qc164" data-title="Parameter Immunoassay Control Set 752 for TBARS" data-catnums="QC164">
QC164, Parameter Immunoassay Control Set 752 for TBARS -
Product Summary
The Parameter TBARS assay is a 2-3 hour chemical analysis designed to measure TBARS in cell culture supernates, cell lysates, serum, plasma, and urine.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 2.57 | 6.07 | 9.26 | 2.39 | 5.71 | 8.76 |
| Standard Deviation | 0.025 | 0.078 | 0.109 | 0.125 | 0.242 | 0.322 |
| CV% | 1 | 1.3 | 1.2 | 5.2 | 4.2 | 3.7 |
Recovery
The recovery of MDA spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 101 | 94-110 |
| Cell Lysis Buffer 3 (n=1) | 99 | 96-103 |
| EDTA Plasma (n=4) | 91 | 89-106 |
| Heparin Plasma (n=4) | 99 | 94-108 |
| Serum (n=4) | 98 | 87-105 |
| Urine (n=4) | 99 | 91-106 |
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of MDA, acid treated, and serially diluted with deionized water to produce samples with values within the dynamic range of the assay.
Preparation and Storage
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Alternate Names
TBARS; Thiobarbituric Acid Reactive Substances;
