Format
96-well strip plate
Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (34 uL), Serum (34 uL), EDTA Plasma (34 uL), Heparin Plasma (34 uL), Urine (10 uL)
Sensitivity
22.4 pg/mL
Assay Range
24.7 - 6,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Specificity
Measures Aldosterone levels in various samples
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Control Available
<div class="add-to-cart" data-type="anc" data-catbase="QC39" data-return="aldosterone-parameter-assay-kit_kge016" data-produrl="parameter-immunoassay-control-set-836-for-aldosterone_qc39" data-title="Parameter Immunoassay Control Set 836 for Aldosterone" data-catnums="QC39">
QC39, Parameter Immunoassay Control Set 836 for Aldosterone -
Product Summary
The Parameter Aldosterone assay is a 3.5 hour competitive enzyme immunoassay designed to measure Aldosterone in cell culture supernates, serum, plasma, and urine.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of kit components.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 278 | 878 | 2536 | 263 | 832 | 2419 |
| Standard Deviation | 9.49 | 17.3 | 34.4 | 31.7 | 83 | 230 |
| CV% | 3.4 | 2 | 1.4 | 12.1 | 10 | 9.5 |
Recovery
The recovery of Aldosterone spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 94 | 89-99 |
| EDTA Plasma (n=4) | 89 | 76-110 |
| Heparin Plasma (n=4) | 89 | 79-107 |
| Serum (n=4) | 88 | 78-108 |
| Urine (n=4) | 101 | 95-106 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Aldosterone were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay as directed in the Sample Preparation section.
Preparation and Storage
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Alternate Names
Aldosterone;
