Species Reactivity
Human
Specificity
Detects human SIRP alpha /CD172a in direct ELISAs and Western blots. In direct ELISAs, 50-100% cross-reactivity with recombinant human (rh) SIRP beta 1 and no cross-reactivity with rhSIRP beta 2 is observed. In Western blots, approximately 10% cross-reactivity with rhSIRP beta 1 and no cross-reactivity with rhSIRP beta 2 is observed.
Source
Monoclonal Mouse IgG2b Clone # 602411
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human SIRP alpha /CD172a
Gly27-Asn370 (predicted)
Accession # P78324Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647
Applications
Recommended
ConcentrationSample
Flow Cytometry
0.25-1 µg/10 6 cells
U937 human histiocytic lymphoma cell line
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: SIRP alpha/CD172a
SIRP alpha (Signal regulatory protein alpha; also SHPS1 and BIT) is a variably glycosylated 90-120 kDa member of the SIRP family of proteins. It is widely expressed, being found on neurons, microglia/macrophages, endothelium, and fibroblasts. SIRP alpha has a variety of functions, including presynsptic organization, inhibition of integrin action, and induction of myogenesis. It binds to CD47 and likely other ligands. Mature human SIRP alpha is a 477 amino acid (aa) type I transmembrane glycoprotein. It contains an extracellular region (aa 27-372) that shows one V-type Ig-like (aa 32-137) and two C2-type Ig-like domains (aa 147-347). Its cytoplasmic domain possesses two ITIMs which interact with protein tyrosine phosphatases. There is one alternative start site at Met102 plus a four aa insertion after Gln421. Over aa 27-370, human SIRP alpha shares 61% aa identity with mouse SIRP alpha.
Long Name:
Signal-regulatory Protein alpha
Entrez Gene IDs:
140885 (Human); 19261 (Mouse); 25528 (Rat); 101926317 (Cynomolgus Monkey)
Alternate Names:
BIT; BITbrain-immunoglobulin-like molecule with tyrosine-based activation motifs; Brain Ig-like molecule with tyrosine-based activation motifs; CD172 antigen-like family member A; CD172a antigen; CD172a; Inhibitory receptor SHPS-1; Macrophage fusion receptor; MFR; MFRtyrosine phosphatase SHP substrate 1; MyD-1 antigen; MYD1; MYD-1; P84; protein tyrosine phosphatase, non-receptor type substrate 1; PTPNS1; SHP substrate 1; SHPS1; SHPS-1; SHPS1CD172A; signal-regulatory protein alpha; Signal-regulatory protein alpha-1; Signal-regulatory protein alpha-2; Signal-regulatory protein alpha-3; SIRP alpha; SIRPA; SIRPalpha; Sirp-alpha-1; SIRPalpha2; Sirp-alpha-2; Sirp-alpha-3; SIRPtyrosine-protein phosphatase non-receptor type substrate 1
