Species Reactivity
Human
Specificity
Detects Integrin alpha 1/CD49a in direct ELISAs.
Source
Monoclonal Rabbit IgG Clone # 1090A
Immunogen
Chinese hamster ovary cell line CHO derived recombinant human Integrin alpha 1 beta 1
Met1-Pro1141 (Integrin alpha 1) & Gln21-Asp728 (Integrin beta 1)
Accession # P56199Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 350
Applications
Recommended
ConcentrationSample
Flow Cytometry
0.25-1 µg/10 6 cells
HeLa human cervical epithelial carcinoma cell line
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Preparation and Storage
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Integrin alpha 1/CD49a
Integrin alpha 1 (also VLA-1, CD49a and Laminin and Collagen Receptor) is a 190-210 kDa member of the integrin alpha chain family of molecules. It is found on smooth muscle cells, osteoblasts, adipocytes and intestinal epithelium. Integrin alpha 1 forms a noncovalent heterodimer with Integrin beta 1, and serves as a divalent-cation dependent receptor for collagen types I, IV, VI, XIII and XVI. It also binds the diarrhea-associated NSP4 enterotoxin of rotavirus. Mature human Integrin alpha 1 is a 1151 amino acid (aa) type I transmembrane glycoprotein that contains a 1113 aa extracellular domain (ECD) and a 15 aa cytoplasmic tail. The ECD contains one vWFA/I-domain (aa 147-360) that binds collagen, plus multiple divalent cation binding sites. Potential splice variants exist that show a two and four aa substitution for aa 765-1179. Over aa 29-1141 (the ECD), human Integrin alpha 1 shares 88% aa identity with mouse Integrin alpha 1.
Entrez Gene IDs:
3672 (Human); 109700 (Mouse); 25118 (Rat)
Alternate Names:
CD49 antigen-like family member A; CD49a antigen; CD49a; Integrin alpha 1; integrin, alpha 1; ITGA1; Laminin and collagen receptor; very late activation protein 1; VLA-1; VLA1integrin alpha-1
