Species Reactivity
Mouse
Specificity
Detects mouse GM-CSF R alpha in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) GM-CSF R alpha or
rhGM-CSF R beta is observed.Source
Monoclonal Rat IgG2a Clone # 698423
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse GM-CSF R alpha
Leu30-Pro327
Accession # Q00941Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647
Applications
Recommended
ConcentrationSample
Flow Cytometry
0.25-1 µg/10 6 cells
J774A.1 mouse reticulum cell sarcoma macrophage cell line
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: GM-CSF R alpha
Granulocyte macrophage colony stimulating factor receptor alpha (GM-CSF R alpha ), also known as CD116, is a component of the receptor complex that mediates cellular responses to GM-CSF. GM-CSF promotes the differentiation and mobilization of granulocyte-macrophage, erythroid, megakaryocyte, and eosinophil progenitors. It enhances the activation of myeloid cell effector functions and plays a role in the development of Th1 biased immune responses, allergic inflammation, and autoimmunity (1-4). Mature mouse GM-CSF R alpha is an 80 kDa type I transmembrane glycoprotein that consists of a 298 amino acid (aa) extracellular domain (ECD) with two fibronectin type III domains and a juxtamembrane WSXWS motif, a 21 aa transmembrane segment, and a 40 aa cytoplasmic domain (5). Within the ECD, mouse GM-CSF R alpha shares approximately 33% and 58% aa sequence identity with human and rat GM-CSF R alpha, respectively. Soluble forms of the human receptor retain the ability to bind GM-CSF (6, 7). GM-CSF R alpha is expressed on hematopoietic stem cells, progenitor and differentiated cells in the myeloid lineage, vascular endothelial cells, placenta, and non‑hematopoietic solid tumor cells (8). GM-CSF R alpha associates with the common beta chain/CD131 ( beta c), a 135 kDa transmembrane protein that is also the signal transducing component of the receptors for IL-3 and IL-5 (9, 10). Association with beta c converts GM-CSF R alpha from a low affinity to a high affinity receptor for GM-CSF (9-11). The shared usage of beta c underlies the synergism between GM-CSF, IL-3, and IL-5 in their effects on myeloid cell differentiation and activation (1, 2).
References:
Martinez-Moczygemba, M. and D.P. Huston (2003) J. Allergy Clin. Immunol. 112:653.
Fleetwood, A.J. et al. (2005) Crit. Rev. Immunol. 25:405.
Eksioglu, E.A. et al. (2007) Exp. Hematol. 35:1163.
Cao, Y. (2007) J. Clin. Invest. 117:2362.
Park, L.S. et al. (1992) Proc. Natl. Acad. Sci. 89:4295.
Pelley, J.L. et al. (2007) Exp. Hematol. 35:1483.
Raines, M.A. et al. (1991) Proc. Natl. Acad. Sci. 88:8203.
Chiba, S. et al. (1990) Cell Regul. 1:327.
Kitamura, T. et al. (1991) Proc. Natl. Acad. Sci. 88:5082.
Hayashida, K. et al. (1990) Proc. Natl. Acad. Sci. 87:9655.
Hoang, T. et al. (1993) J. Biol. Chem. 268:11881.
Long Name:
Granulocyte Macrophage Growth Factor Receptor alpha
Entrez Gene IDs:
1438 (Human); 12982 (Mouse)
Alternate Names:
CD116 antigen; CD116; CDw116; colony stimulating factor 2 receptor alpha subunit; colony stimulating factor 2 receptor, alpha, low-affinity(granulocyte-macrophage); CSF2R; CSF2RA; CSF2RAX; CSF2RAY; CSF2RX; CSF2RY; GM-CSF R alpha; GM-CSF receptor alpha subunit; GMCSFR alpha; GMCSFR; GM-CSFRa; GM-CSF-R-alpha; GMCSFR-alpha; GMR; GMR-alpha; granulocyte-macrophage colony-stimulating factor receptor alpha chain; granulocyte-macrophage colony-stimulating factor receptor subunit alpha; MGC3848; MGC4838
