Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to bind biotinylated Laminarin (1, 3-beta -glucan) in a functional ELISA with an estimated K D <5 nM.
Source
Mouse myeloma cell line, NS0-derived Thr66-Met201, with an N-terminal 10-His tag
Accession #
N-terminal Sequence
AnalysisHis
Predicted Molecular Mass
16.8 kDa
1859-DC |
| |
Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | ||
Reconstitution Reconstitute at 100 μg/mL in sterile PBS. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Dectin-1/CLEC7A
Dectin-1, also known as CLEC7A and the beta -glucan receptor, is a 33 kDa type II transmembrane C-type lectin that participates in the innate immune response to fungal pathogens. Although Dectin-1 structurally resembles other CLEC molecules, it binds its ligands in a calcium-independent manner (1, 2). Mature human Dectin-1 consists of a short N-terminal ITAM-containing cytoplasmic tail, a transmembrane segment, and a C-terminal stalk with a carbohydrate recognition domain (CRD) in the extracellular domain (3, 4). Alternate splicing generates one major splice form that lacks the stalk region (3 - 5). This isoform is expressed on the surface of monocytes, macrophages, myeloid DC, neutrophils, eosinophils, B cells, and CD4+ T cells (6). The CRD selectively binds beta -glucan polymers, a major component of yeast and mycobacterial cell walls (5 - 7). Yeast beta -glucan is accessible to Dectin-1 only during the process of cell budding. Dectin-1 does not recognize the filamentous form of yeast (8). Dectin-1 mediates the phagocytosis of zymosan particles and intact yeast (8 - 10). In the membrane, Dectin-1 colocalizes with TLR2 in the presence of zymosan, and the two receptors cooperate in ligand recognition and the propagation of proinflammatory signaling (9, 11 - 13). Dectin-1 also interacts with tetraspanin CD37. This increases its stability on the cell membrane and inhibits ligand-induced signaling (14). Dectin-1 knockout mice show increased susceptibility to pathogenic infection (15 - 16). The CRD of human Dectin-1 shares 77%, 60%, and 60% amino acid (aa) sequence identity with that of bovine, mouse and rat Dectin-1, respectively. It shares 29% - 39% aa sequence identity with the CRD of other subgroup members, including CLEC-1, CLEC-2, CLEC9A, CLEC12B, LOX-1, and MICL.
References:
Kanazawa, N. (2007) J. Dermatol. Sci. 45:77.
Brown, G.D. (2006) Nat. Rev. Immunol. 6:33.
Hernanz-Falcon, P. et al. (2001) Immunogenetics 53:288.
Yokota, K. et al. (2001) Gene 272:51.
Willment, J.A. et al. (2001) J. Biol. Chem. 276:43818.
Willment, J.A. et al. (2005) Eur. J. Immunol. 35:1539.
Palma, A.S. et al. (2006) J. Biol. Chem. 281:5771.
Gantner, B.N. et al. (2005) EMBO J. 24:1277.
Gantner, B.N. et al. (2003) J. Exp. Med. 197:1107.
Kennedy, A.D. et al. (2007) Eur. J. Immunol. 37:467.
Brown, G.D. et al. (2003) J. Exp. Med. 197:1119.
Yadav, M. and J.S. Schorey (2006) Blood 108:3168.
Suram, S. et al. (2006) J. Biol. Chem. 281:5506.
Meyer-Wentrup, F. et al. (2007) J. Immunol. 178:154.
Saijo, S. et al. (2007) Nat. Immunol. 8:39.
Taylor, P.R. et al. (2007) Nat. Immunol. 8:31.
Entrez Gene IDs:
64581 (Human); 56644 (Mouse)
Alternate Names:
Beta-glucan receptor; BGR; CD369; CLEC7A; CLECSF12; CLECSF12DC-associated C-type lectin 1; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 12; C-type lectin domain family 7 member A; C-type lectin domain family 7, member A; C-type lectin superfamily member 12; Dectin1; Dectin-1; DECTIN1CANDF4; Dendritic cell-associated C-type lectin 1; dendritic cell-associated C-type lectin-1; hDectin-1; lectin-like receptor 1
