Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When 5 x 10 4 cells/well are added to Recombinant Human ICAM-5/Fc Chimera coated plates (25 µg/mL, 100 µL/well), >25% will adhere after 1 hour incubation at 37 °C.
Optimal dilutions should be determined by each laboratory for each application.Source
Mouse myeloma cell line, NS0-derived
Human ICAM-5
(Ala28-Glu570)
Accession # Q9UMF0IEGRMD Human IgG1
(Pro100-Lys330)N-terminus C-terminus Accession #
N-terminal Sequence
AnalysisAla28
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
85.1 kDa (monomer)
SDS-PAGE
125-130 kDa, reducing conditions
1950-M5 |
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Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | ||
Reconstitution Reconstitute at 400 μg/mL in sterile PBS. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: ICAM-5
Intercellular adhesion molecule-5 (ICAM-5), also known as telencephalin, is a cell surface glycoprotein belonging to the immunoglobulin superfamily. Human ICAM-5 consists of an 832 amino acid (aa) extracellular domain containing 9 immunoglobulin (Ig) domains and 15 N-glycosylation sites, a 28 aa transmembrane domain, and a 64 aa cytoplasmic domain. ICAM-5 shares 38 - 55% aa identity with other ICAMs, being most closely related to ICAM-1 (50% identity) and ICAM-3 (55% identity) (1). Human and mouse ICAM-5 share 85% aa identity.
The tissue distribution of ICAM-5 is unique among ICAMs, being expressed only in telencephalic regions of the central nervous system (2). Like other ICAMs, ICAM-5 binds to the leukocyte integrin LFA-1 (CD11a/CD18) (3). Binding of ICAM-5 to LFA-1 is dependent on the first amino terminal Ig domain of ICAM-5 (4). ICAM-5 also displays homophilic binding, with the amino terminal Ig domain binding to Ig domains 4 - 5. Homophilic binding of ICAM-5 is dependent of ICAM-5 being in a monomeric form. The monomeric form of ICAM-5 is found during dendritogenesis in developing brain, whereas a high molecular weight complex is found in mature neurons (5).
References:
Mizuno, T. et al. (1997) J. Biol. Chem. 272:1156.
Yoshihara, Y. et al. (1994) Neuron 12:541.
Tain, L. et al. (1997) J. Immunol. 158:928.
Tain, L. et al. (2000) Eur. J. Immunol. 30:810.
Tain, L. et al. (2000) J. Immunol. 150:243.
Long Name:
Intercellular Adhesion Molecule 5
Entrez Gene IDs:
7087 (Human); 15898 (Mouse)
Alternate Names:
ICAM5; ICAM-5; intercellular adhesion molecule 5; intercellular adhesion molecule 5, telencephalin; Telencephalin; TLCNTelencephalin; TLNtelencephalin
