Recombinant Human Reg1B Protein, CF 50 UG_仪器设备_产品

Purity
>95%, by SDS-PAGE with silver staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using RT4‑D6P2T rat schwannoma cells. The ED ₅₀ for this effect is 0.15-0.75 μg/mL.
Source
E. coli-derived Gln23-Asn166, with an N-terminal Met
Accession #
N-terminal Sequence
Analysis
Met
Predicted Molecular Mass
16 kDa
SDS-PAGE
16 kDa, reducing conditions

2090-RG
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Bioactivity

Recombinant Human Reg1B (Catalog # 2090-RG) stimulates cell proliferation in RT4‑D6P2T rat schwannoma cells. The ED₅₀ for this effect is 0.15-0.75 μg/mL.

SDS-PAGE

1 μg/lane ofRecombinant Human Reg1B (Catalog # 2090-RG)was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditionsand visualized by silver staining, showing bands at 17.2 and 16.7 kDa, respectively.

Background: Reg1B

Reg1B also called Lithostatine-1-beta or Pancreatic stone protein 2 (PSP2) is a type I subclass member of the Reg gene family. The Reg multigene family consists of four subclasses, types I, II, III and IV based on the primary structures of the encoded proteins of the genes. Reg1B is also a member of the Reg family of secreted C-type lectin domain-containing pancreatic proteins (1, 2). The human Reg1B cDNA encodes 166 amino acids protein which contains a 22 aa signal peptide and the 144 aa secreted mature protein. Reg1B shares 86% animo acide sequence identity with Reg1A and like Reg1A is secreted by the exocrine pancreas and associated with islet cell regeneration and diabetogenesis. Reg family proteins were also shown to cause Schwann cell proliferation during regeneration of motor neurons (3).

References:

Liu, J.-L. et al. (2008) Endocrine Metab. Immune Disord. Drug Targets 8:1.
Unno, M. et al. (1993) J. Biol. Chem. 268:15974.
Livesey F.J. et al. (1990) Nature. 390:614.

Long Name:
Regenerating Islet-derived 1B
Entrez Gene IDs:
5968 (Human)
Alternate Names:
Lithostathine 1 beta; lithostathine-1-beta; Pancreatic stone protein 2; pancreatic threadprotein); PSPS2; PSPS2PSP-2; Reg1B; regenerating islet-derived 1 beta; Regenerating islet-derived protein 1-beta; Regenerating protein I beta; REGH; REGLREG-1-beta; secretory pancreatic stone protein 2