Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to enhance survival of E16-E18 rat embryonic cortical neurons.
The ED50 for this effect is 0.6-2.4 μg/mL.
Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Optimal neurite outgrowth is observed between 1.25-10 μg/mL.
Source
Mouse myeloma cell line, NS0-derived Gly28-His367, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
AnalysisStarts at Gly28
Predicted Molecular Mass
39.5 kDa
SDS-PAGE
50-65 kDa, reducing conditions
2565-AM |
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Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | ||
Reconstitution Reconstitute at 100 μg/mL in PBS. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: AMIGO
AMIGO (amphoterin-induced gene and ORF) is an approximately 75 kDa transmembrane cell adhesion protein. It belongs to a family of leucine‑rich repeat (LRR) containing proteins that play various roles in nervous system development and function (1). Mature human AMIGO consists of a 345 amino acid (aa) extracellular domain (ECD) with six tandem LRRs flanked by LRR N- and C‑terminal domains, one immunoglobulin‑like domain, a 21 aa transmembrane segment, and a 100 aa cytoplasmic domain (2). Within the ECD, human AMIGO shares 86% aa sequence identity with mouse and rat AMIGO. AMIGO is an N‑glycosylated protein that forms homodimers by association between LRR regions (3, 4). It interacts homophilically as well as heterophilically with the related AMIGO2 and AMIGO3 proteins (2). AMIGO is expressed along developing and mature neuronal fiber tracts on neurons, astrocytes, and oligodendrocytes (2, 4). It promotes the extension and branching of hippocampal and cortical neurons (2, 4). It also supports neuronal survival following apoptotic stimulation (4). AMIGO directly associates with the potassium channel Kv2.1 and enhances ion conductance through the channel (5). Kv2.1 localization and activation is regulated by phosphorylation, but phosphorylation status does not alter its interaction with AMIGO (5, 6).
References:
de Wit, J. et al. (2011) Annu. Rev. Cell Dev. Biol. 27:697.
Kuja-Panula, J. et al. (2003) J. Cell Biol. 160:963.
Kajander, T. et al. (2011) J. Mol. Biol. 413:1001.
Chen, Y. et al. (2012) J. Cell. Physiol. 227:2217.
Peltola, M.A. et al. (2011) EMBO Rep. 12:1293.
Park, K.-S. et al. (2006) Science 313:976.
Long Name:
Amphoterin-induced Gene and ORF
Entrez Gene IDs:
57463 (Human); 229715 (Mouse)
Alternate Names:
adhesion molecule with Ig-like domain 1; ali2; Alivin-2; AMIGO; AMIGO1; AMIGO-1; AMIGOamphoterin-induced protein 1; amphoterin-induced gene and ORF; KIAA1163
