Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons. Able to significantly inhibit neurite outgrowth when immobilized as a 3 μL droplet containing 90 ng on a nitrocellulose coated microplate.
Source
Mouse myeloma cell line, NS0-derived Ser1583-Ser2224, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
AnalysisSer1583
Predicted Molecular Mass
69 kDa
SDS-PAGE
100-110 kDa, reducing conditions
2585-PG |
| |
Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | ||
Reconstitution Reconstitute at 300 μg/mL in PBS. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: NG2/MCSP
NG2, also known as CSPG4, MCSP, and AN2, is a 400-500 kDa transmembrane chondroitin sulfate proteoglycan (CSPG) with a protein core of approximately 300 kDa. The extracellular region can be proteolytically shed from the cell surface (1-3). Mature human NG2 consists of a 2195 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 77 aa cytoplasmic domain (4). Within aa 1583-2224, human NG2/CSPG4 shares 83% aa sequence identity with mouse and rat CSPG4. NG2 binds to the extracellular matrix proteins Laminin, Tenascin, and Collagens II, V, and VI as well as to the growth factors FGF-2 and PDGF-AA (1, 5, 6). NG2 is expressed on glial cell progenitors known as O2A cells or NG2 glia (2, 7, 8). These cells are neuronally responsive and differentiate primarily into oligodendrocytes but also into astrocytes (8-10). NG2 associates with PDGF R alpha and the AMPA R subunit GluR2 (2, 7). It is up-regulated on microglial cells during inflammation and contributes to the induction of inflammatory mediators (11). Various CSPGs in the brain inhibit neurite outgrowth through interactions with Nogo Receptor/NgR1 and NgR3 (12). This recombinant protein product corresponds to the last 5 CSPG repeats, a region which can independently inhibit neurite outgrowth (13). NG2 is also expressed on vascular mural cells and capillaries (10, 14). It promotes vascular endothelial cell (EC) migration and angiogenesis through interactions with Galectin-3 and Integrin alpha 3 beta 1 on EC, Plasminogen, and Angiostatin (15, 16). NG2 is also expressed on a variety of tumors where it contributes to tumor cell adhesion, motility, and invasion (17).
References:
Campoli, M. et al. (2010) Adv. Cancer Res. 109:73.
Nishiyama, A. et al. (1996) J. Neurosci. Res. 43:315.
Nishiyama, A. et al. (1995) Mol. Biol. Cell 6:1819.
Pluschke, G. et al. (1996) Proc. Natl. Acad. Sci. USA 93:9710.
Burg, M.A. et al. (1996) J. Biol. Chem. 271:26110
Goretzki, L. et al. (1999) J. Biol. Chem. 274:16831.
Stegmuller, J. et al. (2003) J. Biol. Chem. 278:3590.
Komitova, M. et al. (2009) J. Comp. Neurol. 512:702.
Bergles, D.E. et al. (2000) Nature 405:187.
Zhu, X. et al. (2008) Development 135:145.
Gao, Q. et al. (2010) Neuroscience 165:386.
Dickendesher, T.L. et al. (2012) Nat. Neurosci. 15:703.
Ughrin, Y.M. et al. (2003) J. Neurosci. 23:175.
Schlingemann, R.O. et al. (1990) Am. J. Pathol. 136:1393.
Fukushi, J. et al. (2004) Mol. Biol. Cell 15:3580.
Goretzki, L. et al. (2000) J. Biol. Chem. 275:28625.
Wang, X. et al. (2010) Curr. Mol. Med. 10:419.
Long Name:
Chondroitin Sulfate Proteoglycan NG2/Melanoma Associated Chondroitin Sulfate Proteoglycan
Entrez Gene IDs:
1464 (Human); 121021 (Mouse); 81651 (Rat)
Alternate Names:
AN2; chondroitin sulfate proteoglycan 4 (melanoma-associated); chondroitin sulfate proteoglycan 4; CSPG4; EC 3.6.3; HMW-MAA; MCSP; MCSPChondroitin sulfate proteoglycan NG2; MCSPG; MCSPGMelanoma chondroitin sulfate proteoglycan; MEL-CSPG; MSK16Melanoma-associated chondroitin sulfate proteoglycan; NG2; NG2EC 2.7.8
