Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized rhGas1 at 1 µg/mL (100 µL/well) can bind rmArtemin at a linear range of 0.1-10 ng/mL.
Source
Mouse myeloma cell line, NS0-derived Leu40-Ser318, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
AnalysisLeu40
Structure / Form
Monomer
Predicted Molecular Mass
30.1 kDa
SDS-PAGE
40 kDa, reducing conditions
2636-GS |
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Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | ||
Reconstitution Reconstitute at 100 μg/mL in sterile PBS. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: Gas1
Gas1 (Growth Arrest Specific 1) is one of six structurally unrelated proteins that were identified by their increased expression in growth-arrested cells relative to actively proliferating cells (1, 2). Following mitogenic stimulation, Gas1 expression is transcriptionally suppressed by c-Myc as cells transit from G0 to G1 phases of the cell cycle (3, 4). Overexpression of Gas1 prevents S phase entry and DNA synthesis (5). Gas1-mediated blockade of the cell cycle is p53-dependent but does not require the transactivating domain of p53 (6). The human Gas1 cDNA encodes a 345 amino acid (aa) precursor that includes a 39 aa signal sequence, a 279 aa mature protein, and a 27 aa C-terminal propeptide. Gas1 contains Ala-rich and Asp-rich regions as well as an RGD sequence (5). Mature human and mouse Gas1 share 85% aa sequence identity. Human Gas1 is a 40 kDa GPI-linked glycoprotein that is uniformly distributed on the cell surface (7). In contact-inhibited vascular endothelial cells, Gas1 is induced by VE-Cadherin and VEGF expression and mediates the anti-apoptotic effect of VEGF (8). In contrast, Gas1 is induced in hippocampal neurons after NMDA exposure but functions as a pro-apoptotic effector of NMDA-mediated excitotoxicity (9). Gas1 exhibits a range of developmental actions including either promoting or inhibiting growth and differentiation of somite, limb, cerebellar, and eye tissues (10 - 14). Gas1 contributes to the antagonistic effect of Wnt proteins toward Shh function by binding the N-terminal region of Shh (11). The dependence of Gas1 function on the cellular context has been addressed by suggesting that Gas1 could function as a co-receptor for GDNF family ligands (15). This speculation is supported by R&D Systems data which demonstrate direct binding of Gas1 to Artemin and Neurturin.
References:
Schneider, C. et al. (1988) Cell 54:787.
Mullor, J.L. and A.R. Altaba (2002) BioEssays 24:22.
Del Sal, G. et al. (1994) Proc. Natl. Acad. Sci. USA 91:1848.
Lee, T.C. et al. (1997) Proc. Natl. Acad. Sci. USA 94:12886.
Del Sal, G. et al. (1992) Cell 70:595.
Del Sal, G. et al. (1995) Mol. Cell. Biol. 15:7152.
Stebel, M. et al. (2000) FEBS Lett. 481:152.
Spagnuolo, R. et al. (2004) Blood 103:3005.
Mellstrom, B. et al. (2002) Mol. Cell Neurosci. 19:417.
Lee, K.K.H. et al. (2001) Dev. Biol. 234:188.
Lee, C.S. et al. (2001) Proc. Natl. Acad. Sci. USA 98:11347.
Liu, Y. et al. (2002) Development 129:5289.
Liu, Y. et al. (2001) Dev. Biol. 236:30.
Lee, C.S. et al. (2001) Dev. Biol. 236:17.
Schueler-Furman, O. et al. (2006) Trends Pharmacol. Sci. 27:72.
Long Name:
Growth-arrest-specific Protein 1
Entrez Gene IDs:
2619 (Human); 14451 (Mouse)
Alternate Names:
Gas1; GAS-1; growth arrest-specific 1; Growth arrest-specific gene-1; growth arrest-specific protein 1
