Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of HT‑29 human colon adenocarcinoma cells. When 5 x 10 4 cells/well are added to rhLAIR-2 coated plates (50 µg/mL with
100 µL/well), approximately 40%-60% will adhere after 10 minutes at 37° C.
Optimal concentration depends on cell type as well as the application or research objectives.Source
Mouse myeloma cell line, NS0-derived Gln22-Pro152, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
AnalysisNo results obtained: Gln22 predicted & Thr37
Predicted Molecular Mass
14.9 kDa
SDS-PAGE
20 kDa, reducing conditions
2665-LR |
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Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | ||
Reconstitution Reconstitute at 100 μg/mL in sterile PBS. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: LAIR2
LAIR-2 (leukocyte-associated Ig-like receptor-2; CD306) is a secreted, 131 amino acid (aa) protein that contains one Ig-like C2 type domain, making it a member of the Ig superfamily. When compared to LAIR-1, its transmembrane counterpart, it shares 83% aa identity across the signal sequence and extracellular domains; (1-3) although one is secreted and one is membrane-bound, the two LAIR proteins are thought to have arisen from a common gene ancestor and appear to share similar adhesion profiles. This suggests that LAIR-2 may compete with LAIR-1 for ligand binding (3, 4). A 114 aa alternate splice form of LAIR-2 is truncated at the C terminus, but retains the entire Ig domain (1-3). The expression profile of these splice forms, and the presence of orthologs in other species, have not been reported.
References:
Meyaard, L. (2003) J. Biol. Regul. Homeost. Agents 17:330.
Meyaard, L. et al. (1999) J. Immunol. 162:5800.
Meyaard, L. et al. (1997) Immunity 7:283.
Xu, X.G. et al. (2005) Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 21:553.
Long Name:
Leukocyte-associated Ig-like Receptor 2
Entrez Gene IDs:
3904 (Human)
Alternate Names:
CD306 antigen; CD306; CD306leukocyte-associated Ig-like receptor-2; LAIR2; LAIR-2; leukocyte-associated Ig-like receptor 2; leukocyte-associated immunoglobulin-like receptor 2; MGC71634
