Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of the A549 human lung carcinoma cells. The ED 50 for this effect is 0.15-0.75 μg/mL.
Source
E. coli-derived Phe21-Gly104
Accession #
N-terminal Sequence
AnalysisPhe21
Predicted Molecular Mass
8.2 kDa
SDS-PAGE
6 kDa, reducing conditions
2790-HN |
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Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | ||
Reconstitution Reconstitute at 250 μg/mL in PBS. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: HIN-1/SCGB3A1
High in Normal‑1 (HIN‑1), also known as Uteroglobin related protein 2 (UGRP2), is an 8 kDa secreted protein of the secretoglobin superfamily (SCGB3A1) (1). HIN‑1 is expressed in bronchial epithelial and secretory Clara cells, mammary epithelial cells (particularly during pregnancy), salivary glands, and the prostate (2‑5). It is up‑regulated in Clara cells by IL‑4, IL‑13, Oncostatin M, EGF, and TGF‑ alpha and down‑regulated by IFN‑ alpha, beta, and gamma (6‑9). It is secreted into bronchial lavage fluid, saliva, and plasma, and may form disulfide‑linked dimers (10). HIN‑1 binds to the macrophage scavenger receptor MARCO, and to the surface of mammary and bronchial epithelial cells (11, 12). HIN‑1 promotes apoptosis and inhibits the proliferation, migration, and invasion of breast cancer cells (12). The down‑regulation of HIN‑1 expression in many breast, lung, and prostate cancers correlates with hypermethylation of its promoter (4, 10, 13, 14). Mature human HIN‑1 shares 58% and 62% aa sequence identity with mouse and rat HIN‑1, respectively (4).
References:
Mukherjee, A.B. et al. (2007) Endocr. Rev. 28:707.
Porter, D. et al. (2002) Mech. Dev. 114:201.
Reynolds, S.D. et al. (2002) Am. J. Respir. Crit. Care Med. 166:1498.
Krop, I. et al. (2001) Proc. Natl. Acad. Sci. 98:9796.
Niimi, T. et al. (2002) Cytogenet. Genome Res. 97:120.
Yamada, A. et al. (2005) J. Immunol. 175:5708.
Tomita, T. et al. (2009) Am. J. Respir. Cell Mol. Biol. 40:620.
Yamada, A. and S. Kimura (2005) FEBS Lett. 579:2221.
Yamada, A. et al. (2009) Biochem. Biophys. Res. Commun. 379:964.
Krop, I. et al. (2004) Mol. Cancer Res. 2:489.
Bin, L.-H. et al. (2003) J. Immunol. 171:924.
Krop, I. et al. (2005) Cancer Res. 65:9659.
Krop, I. et al. (2003) Cancer Res. 63:2024.
Shigematsu, H. et al. (2005) Int. J. Cancer 113:600.
Long Name:
High in Normal 1
Entrez Gene IDs:
92304 (Human); 73945 (Mouse)
Alternate Names:
cytokine high in normal-1; Cytokine HIN-1; High in normal 1; HIN1; HIN-1; HIN1Pneumo secretory protein 2; LU105; PNSP2; PnSP-2; PnSP-2Uteroglobin-related protein 2; SCGB3A1; secretoglobin family 3A member 1; secretoglobin, family 3A, member 1; UGRP2; UGRP2MGC87867
