Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When rhNectin-3 is coated at 1 μg/mL, rhNectin-1 binds with an apparent K D <25 nM.
Source
Mouse myeloma cell line, NS0-derived Gln31-Thr334 with a C-terminal 6-His tag
Accession #
N-terminal Sequence
AnalysisNo results obtained: Gln31 predicted
Predicted Molecular Mass
35.0 kDa
SDS-PAGE
50-65 kDa, reducing conditions
2880-N1 |
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Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | ||
Reconstitution Reconstitute at 300 μg/mL in PBS. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: Nectin-1
Nectin-1 (designated CD111), also called PRR-1 (poliovirus receptor-related protein 1) or HVEC (herpesvirus entry mediator C), is a widely expressed 110 kDa type I transmembrane glycoprotein important in formation of adherens junctions and synapses. It is a member of the nectin family within the Ig superfamily (1, 2). The Latin word necto means “to connect”, indicating the role of nectins in Ca2+-independent cell-cell adhesion (2). Nectin-1 forms homodimers in cis, followed by interactions in trans with Nectin-1, -3 or -4 (2). The 517 amino acid (aa) human Nectin-1 isoform 1 contains a 30 aa signal sequence, a 325 aa extracellular domain (ECD), a 21 aa transmembrane segment (TM), and a 141 aa cytoplasmic region. Nectin ECDs contain three Ig-like domains: an N-terminal V-type that mediates ligand binding, and two C2-type (3). Nectin-1, like other nectins, has a splice form (isoform 2 or HigR, 458 aa) with alternate TM and cytoplasmic sequences. Another, isoform 3, is a 352 aa secreted protein (4). The common region of mature human Nectin-1 (aa 31 - 334) shares 93%, 94%, 96% and 96% aa identity with mouse, rat, bovine and porcine Nectin-1, respectively. Nectin-1 binds viral glycoprotein D to mediate herpesvirus (but not poxvirus) entry into vaginal mucosa, sensory neurons and fibroblasts (4 - 7). In forming adherens junctions and synapses, nectins 1 and 3 initiate cell-cell interactions, recruiting alpha v beta 3 integrin extracellularly and cadherins intracellularly through afadin and other junctional proteins (2, 8 - 11). These interactions organize the cytoskeleton, strengthen attachment to basement membrane and promote further cell-cell connections. Nectin-1 also recognizes CD96 on NK cells (12). Deficiency of Nectin-1 can result in cleft lip/palate ectodermal dysplasia (13). Nectin-1 downregulation in epithelial cancers, mediated in part by ectodomain shedding, may contribute to invasiveness (14).
References:
Lopez, M. et al. (1995) Gene 155:261.
Takai, Y. et al. (2008) Nat. Rev. Mol. Cell Biol. 9:603.
Fabre, S. et al. (2002) J. Biol. Chem. 277:27006.
Lopez, M. et al. (2001) J. Virol. 75:5684.
Cocchi, F. et al. (1998) Proc. Natl. Acad. Sci. USA 95:15700.
Linehan, M. M. et al. (2004) J. Virol. 78:2530.
Simpson, S. A. et al. (2005) J. Neurovirol. 11:208.
Mizoguchi, A. et al. (2002) J. Cell Biol. 156:555.
Togashi, H. et al. (2006) J. Cell Biol. 174:141.
Tachibana, K. et al. (2000) J. Cell Biol. 150:1161.
Takai, Y. and H. Nakanishi (2003) J. Cell Science 116:17.
Seth, S. et al. (2007) Biochem. Biophys. Res. Commun. 364:959.
Suzuki, K. et al. (2000) Nat. Genet. 25:427.
Tanaka, Y. et al. (2002) Biochem. Biophys. Res. Commun. 299:472.
Long Name:
Poliovirus Receptor Related 1
Entrez Gene IDs:
5818 (Human)
Alternate Names:
CD111 antigen; CD111; CLPED1ectodermal dysplasia 4 (Margarita Island type); ED4; Herpes virus entry mediator C; Herpesvirus entry mediator C; Herpesvirus Ig-like receptor; HIgRPVRR; HVEC; HVECpoliovirus receptor-like 1; MGC142031; Nectin1; Nectin-1; OFC7nectin 1; poliovirus receptor-related 1 (herpesvirus entry mediator C); poliovirus receptor-related protein 1; PRR; PRR1; PRR1MGC16207; PVRL1; PVRR1; PVRR1nectin-1; SK-12
