Purity
>95%, by SDS-PAGE with silver staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to neutralize Activin-mediated erythroid differentiation of K562 human chronic myelogenous leukemia cells. The ED 50 for this effect is 5-30 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived Ser234-Ile366 ( alpha chain) & Gly311-Ser426 ( beta chain)
Accession #
N-terminal Sequence
AnalysisSer234 ( alpha chain) & Gly311 ( beta chain)
Structure / Form
Disulfide-linked heterodimer
Predicted Molecular Mass
14.5 kDa ( alpha chain) & 13.0 kDa ( beta chain)
SDS-PAGE
12-14 kDa & 17-20 kDa, reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
8346-IN |
| 8346-IN/CF |
Formulation Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein. | Formulation Lyophilized from a 0.2 μm filtered solution in HCl. | |
Reconstitution Reconstitute at 100 μg/mL in 4 mM HCl. | Reconstitution Reconstitute at 100 μg/mL in 4 mM HCl. | |
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
| Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Data Images
| Recombinant Mouse Inhibin A (Catalog # 8346-IN) neutralizes Activin-mediated erythroid differentiation of K562 human chronic myelogenous leukemia cells. The ED50 for this effect is 5-30 ng/mL. |
Background: Inhibin A
Inhibin A is a member of the TGF-beta superfamily of proteins (1). Mature Inhibin A is a disulfide-linked dimer composed of alpha and beta subunits (1). Inhibin A shares a common alpha subunit with the closely related protein Inhibin B but has a unique beta subunit ( beta A) (2-4). The mature alpha subunit of mouse Inhibin A has a predicted molecular weight of 14.7 kDa and shares 80% and 97% amino acid (aa) sequence identity with the human and rat orthologs, respectively. The mature beta subunit of this mouse protein has a predicted molecular weight of 13 kDa and shares 93% and 100% aa sequence identity with the human and rat orthologs, respectively. Inhibin binds and antagonizes ActRIIA and ActRIIB in complex with the TGF-beta RIII and/or IGSF1 co-receptors and subsequently acts to suppress Activin-induced Follicle Stimulating Hormone (FSH) secretion (1, 5-7). Inhibins are produced by gonadal cells in both males and females (8). They are thought to be involved in the regulation of gametogenesis, and embryonic and fetal development (8, 9). Elevated concentrations of Inhibins are associated with pregnancy, preeclampsia, and ovarian cancer, and Inhibin A levels are typically measured during prenatal screening for Down’s syndrome (10-14).
References:
Phillips, D.J. and T.K. Woodruff (2004) Growth Factors 22:13.
Ling, N. et al. (1985) Proc. Natl. Acad. Sci. USA 82:7217.
Robertson, D.M. et al. (1985) Biochem. Biophys. Res. Commun. 126:220.
Mason, A.J. et al. (1986) Biochem. Biophys. Res. Commun. 135:957.
Lewis, K.A. et al. (2000) Nature 404:411.
Martens, J.W. et al. (1997) Endocrinology 138:2928.
Chapman, S.C. et al. (2002) Mol. Cell. Endocrinol. 196:79.
de Kretser, D.M. and D.M. Robertson (1989) Biol. Reprod. 40:33.
Knight, P.G. et al. (2012) Mol. Cell. Endocrinol. 359:53.
Walentowicz, P. et al. (2014) PLoS One 9:e90575.
Kondi-Pafiti, A. et al. (2013) Clin. Exp. Obstet. Gynecol. 40:109.
Kuijper, E.A. et al. (2013) Reprod. Biomed. Online 27:33.
Carty, D.M. et al. (2008) Trends Cardiovasc. Med. 18:186.
Wald, N.J. et al. (1996) Prenat. Diagn. 16:143.
Entrez Gene IDs:
3623 (Human); 16322 (Mouse)
Alternate Names:
A-inhibin subunit; INHA; Inhibin A; inhibin alpha chain; inhibin, alpha
