Purity
>90%, by SDS-PAGE with silver staining
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse Integrin alpha V beta 8 at 2 μg/mL can bind Recombinant Human LAP (TGF-beta 1) (Catalog # ) with an apparent K d <0.1 nM.
Source
Chinese Hamster Ovary cell line, CHO-derived
Mouse Integrin aV
(Phe31-Val988)
Accession # P43406His-Pro GGGSGGGS Acidic Tail 6-His tag Mouse Integrin beta 8
(Glu43-Ser679)
Accession # Q0VBD0His-Pro GGGSGGGS Basic Tail N-terminus C-terminus Accession #
N-terminal Sequence
AnalysisPhe31 ( alpha V subunit) & Glu43 ( beta 8 subunit)
Structure / Form
Non-covalent heterodimer
Predicted Molecular Mass
115 kDa ( alpha V subunit), 78 kDa ( beta 8 subunit)
SDS-PAGE
95 - 170 kDa, reducing conditions
8314-AV |
| |
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and Trehalose. | ||
Reconstitution Reconstitute at 500 μg/mL in sterile PBS. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Integrin alpha V beta 8
Integrin alpha V beta 8 is a type I transmembrane non-covalent heterodimer composed of a 115 kDa alpha V/CD51 subunit and an 84 kDa beta 8 subunit. The Integrin beta 8 exclusively partners with the alpha V subunit, while alpha V forms heterodimers with beta 1, beta 3, beta 5 and beta 6 (1). Integrin alpha V beta 8 is expressed on Schwann cells and astrocytes in the brain, vascular epithelial cells, mesangial cells in the kidney, and fibroblasts and epithelial cells in the airway (2-7). Interestingly, Integrin alpha V beta 8 is expressed by cells of the immune system, most prominently on CD4 + T cells and on dendritic cells (8). Unlike other alpha V integrins, alpha V beta 8 does not interact with the cytoskeleton or activate cytoplasmic signaling pathways (3, 9). Instead, it binds ligands containing an arginine-glycine-aspartic acid (RGD) motif, including Vitronectin, Fibrin and the latency associated peptide (LAP) (10, 11). High affinity binding of alpha V beta 8 to LAP triggers the MT1-MMP induced proteolytic cleavage of LAP and the release of active TGF-beta (12). Active TGF-beta regulates cell growth and nearby vascularization (5, 12, 13). Furthermore, Integrin alpha V beta 8-mediated TGF-beta activation in specialized dendritic cells of the intestine is crucial for maintaining immune homeostasis in the gut (14). Deletion of either alpha V or beta 8 reveals that alpha V beta 8 is required for vascular morphogenesis in the embryonic brain (15-16). The 958 amino acid mouse alpha V extracellular domain (ECD) shares 92% and 88% aa sequence identity with human and rat alpha V, respectively, while the 637 aa mouse beta 8 ECD shares 87% and 96% aa sequence identity with human and rat orthologs, respectively.
References:
Luo, BH. et al. (2007) Annu.Rev.Imm.25:619.
Milner, R. et al. (1997) Glia 21:350.
Nishimura, S. et al. (1998) Brain Res. 791:271.
Zhu, J. et al. (2002) Development 129:2891.
Cambier, S. et al. (2000) Cancer Res. 60:7084.
Khan, S. et al. (2011) Am. J. Pathol. 178:609.
Araya, J. et al. (2006) Am. J. Pathol. 169:405.
Travis, M.A. et al. (2007) Nature 449:361.
Moyle, M. et al. (1991) J. Biol. Chem. 266:19650.
Nishimura, S. et al. (1994) J. Biol. Chem. 269:28708.
Chernousov, M. A. and D. J. Carey (2003) Exp. Cell Res. 291:514.
Mu, D. et al. (2002) J. Cell Biol. 157:493.
Araya, J. et al. (2006) Am. J. Pathol. 169:405.
Worthington, J.J. et al. (2012) Immunobiology 217:1259.
Cambier, S. et al. (2005) Am. J. Pathol. 166:1883.
Proctor, J. M. et al. (2005) J. Neurosci. 25:9940.
McCarty, J. H. et al. (2005) Development 132:165.
Entrez Gene IDs:
3685 (Human)
Alternate Names:
Integrin alpha V beta 8
