Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The ED 50 for this effect is 0.03-0.3 ng/mL.
Source
Spodoptera frugiperda, Sf 21 (stably transfected)-derived Gln19-Ile144
Accession #
N-terminal Sequence
AnalysisNo results obtained. Gln19 inferred from enzymatic pyroglutamate treatment revealing Gly20
Predicted Molecular Mass
14 kDa
SDS-PAGE
12-24 kDa, reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
209-ILB |
| 209-ILB/CF |
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. | Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | |
Reconstitution Reconstitute at 100 μg/mL in PBS. | Reconstitution Reconstitute at 100 μg/mL in PBS. | |
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
| Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Data Images
Bioactivity
| Recombinant Human IL-9 (Catalog # 209-ILB) induces MO7ehuman megakaryocytic leukemic cell proliferation. The ED50 for this effect is 0.03-0.3 ng/mL. |
Background: IL-9
Interleukin-9 (IL-9), also known as P40 and MEA (mast cell growth-enhancing activity), is a 30-40 kDa glycosylated member of a cytokine family that includes Interleukins-2, -4, -7, -15, and -21. These proteins utilize heteromeric receptors containing the Common gamma chain ( gamma c) in addition to ligand-specific subunits. IL-9 interacts selectively with IL-9 R which then associates with gamma c to form the functional receptor complex. IL-9 contributes to allergic inflammation, autoimmunity-induced inflammation, parasite clearance from the GI tract, and Treg-mediated immune suppression (1, 2). It enhances the expansion and recruitment of mast cells and eosinophils as well as the production of IgE and Th2 cytokines (3‑6). It is required for anaphylactic responses to ingested allergens but not to systemic allergens (7). IL-9 plays multiple roles in the development and function of subsets within the CD4 + T cell lineage (8). It is expressed by activated Th9, Th17, Treg, and Th2 cells (3, 9‑12). IL-9 acts as an autocrine growth and activation factor for Th17, Treg, and mast cells (3, 11, 13). It also can inhibit immune responses by enhancing the suppressive properties of Treg and by recruiting immune-suppressive mast cells to sites of inflammation (11, 12). Mature human IL-9 shares 57% amino acid sequence identity with mouse and rat IL-9 (14, 15).
References:
Noelle, R.J. and E.C. Nowak (2010) Nat. Rev. Immunol. 10:683.
Goswami, R. and M.H. Kaplan (2011) J. Immunol. 186:3283.
Nowak, E.C. et al. (2009) J. Exp. Med. 206:1653.
Townsend, M.J. et al. (2000) Immunity 13:573.
Leech, M.D. and R.K. Grencis (2006) J. Immunol. 176:2505.
Fawaz, L.M. et al. (2007) J. Allergy Clin. Immunol. 120:1208.
Osterfeld, H. et al. (2010) J. Allergy Clin. Immunol. 125:469.
Jabeen, R. and M.H. Kaplan (2012) Curr. Opin. Immunol. 24:303.
Tan, C. et al. (2010) J. Immunol. 185:6795.
Jager, A. et al. (2009) J. Immunol. 183:7169.
Elyaman, W. et al. (2009) Proc. Natl. Acad. Sci. USA 106:12885.
Lu, L.-F. et al. (2006) Nature 442:997.
Stassen, M. et al. (2000) J. Immunol. 164:5549.
Yang, Y.-C. et al. (1989) Blood 74:1880.
Renauld, J.-C. et al. (1990) Cytokine 2:9.
Long Name:
Interleukin 9
Entrez Gene IDs:
3578 (Human); 16198 (Mouse)
Alternate Names:
Cytokine P40; HP40; IL9; IL-9; IL-9homolog of mouse T cell and mast cell growth factor 40; interleukin 9; interleukin-9; p40 cytokine; p40 T-cell and mast cell growth factor; P40; T-cell growth factor P40
