Species Reactivity
Human
Specificity
Detects human LIGHT/TNFSF14 in ELISAs. In sandwich immunoassays, no significant cross-reactivity or interference with recombinant human (rh) Fas Ligand, rhAPRIL, recombinant mouse TRANCE, rhTNF-alpha or rhTRAIL is observed.
Source
Monoclonal Mouse IgG 1 Clone # 115520
Purification
Protein A or G purified from ascites
Immunogen
Mouse myeloma cell line NS0-derived recombinant human LIGHT/TNFSF14
Asp74-Val240
Accession # O43557Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin
Applications
Recommended
ConcentrationSample
Flow Cytometry
10 µL/10 6 cells
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Flow Cytometry | Detection of LIGHT/TNFSF14 in Human CD3+ T cells by Flow Cytometry. Human CD3+ T cells treated with 1 ng/mL PMA and 1 μg/mL Calcium Ionomycin for 36 hours were stained with Mouse Anti-Human LIGHT/TNFSF14 PE‑conjugated Monoclonal Antibody (Catalog # FAB664P, filled histogram) or isotype control antibody (Catalog # , open histogram). Cells were gated on CD4+ cells using Mouse Anti-Human CD4 APC-conjugated Monoclonal Antibody (Catalog # ). View our protocol for . |
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: LIGHT/TNFSF14
Human LIGHT, also known as TNFSF14, is a type II membrane protein that is a member of the TNF superfamily. LIGHT is an acronym which stands for "is homologous to lymphotoxins, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes". LIGHT has also been called HVEM-L and LT-gamma. LIGHT is a 240 amino acid (aa) protein that contains a 37 aa cytoplasmic domain, a 22 aa transmembrane region, and a 181 aa extracellular domain. Similar to other TNF ligand family members, LIGHT is predicted to assemble as a homotrimer. LIGHT is produced by activated T cells and was first identified by its ability to compete with HSV glycoprotein D for HVEM binding. LIGHT has also been shown to bind to the lymphotoxin beta receptor (LT beta R) and the decoy receptor (DcR3/TR6). LIGHT overexpression in tumor cells induces apoptosis, which can be enhanced by IFN-gamma.
References:
Mauri, D.N. et al. (1998) Immunity 8:21.
Zhai, Y. et al. (1998) J. Clin. Invest. 102:1142.
Harrop, J.A. et al. (1998) J. Biol. Chem. 273:27548.
Yu, K-Y. et al. (1999) J. Biol. Chem. 274:13733.
Long Name:
TNF Ligand Superfamily Member 14
Entrez Gene IDs:
8740 (Human); 50930 (Mouse)
Alternate Names:
CD258 antigen; CD258; delta transmembrane LIGHT; Herpes virus entry mediator ligand; Herpesvirus entry mediator ligand; HVEM-L; HVEMLherpesvirus entry mediator-ligand; ligand for herpesvirus entry mediator; LIGHT; LIGHTherpesvirus entry mediator A; LTg; TNFSF14; TR2; tumor necrosis factor (ligand) superfamily, member 14; tumor necrosis factor ligand superfamily member 14; tumor necrosis factor receptor-like 2; tumor necrosis factor superfamily member LIGHT
