Species Reactivity
Human, Mouse
Specificity
Detects human and mouse Sonic Hedgehog/SHH in Western blots and mouse Sonic Hedgehog/SHH in ELISAs. In Western blots, approximately 50% cross-reactivity with recombinant mouse (rm) Desert Hedgehog/Dhh (N‑terminus; aa 23-198) is observed and no cross-reactivity with rmIndian Hedgehog/Ihh (N-terminus; aa 66-240), rmDesert Hedgehog/Dhh (C‑terminus; aa 199-396), or rmSonic Hedgehog/Shh (C‑terminus; aa 199-437) is observed.
Source
Monoclonal Rat IgG 2A Clone # 171018
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse Sonic Hedgehog/SHH N-terminus
Cys25-Gly198 (Lys122Arg)
Accession # Q62226Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin
Applications
Recommended
ConcentrationSample
Intracellular Staining by Flow Cytometry
10 µL/10 6 cells
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry | Detection of Sonic Hedgehog/Shh in PC‑3 Human Cell Line by Flow Cytometry. PC‑3 human prostate cancer cell line was stained with Rat Anti-Human/Mouse Sonic Hedgehog/Shh N-Terminus PE‑conjugated Monoclonal Antibody (Catalog # IC4641P, filled histogram) or isotype control antibody (Catalog # , open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # ) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # ). View our protocol for . |
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: Sonic Hedgehog/Shh
Sonic Hedgehog (Shh) is a hedgehog protein that is instrumental in patterning the early embryo. The N-terminal peptide of Shh is released by autoproteolysis and functions through interactions with a multicomponent receptor complex containing the transmembrane proteins Patched and Smoothened.
Entrez Gene IDs:
6469 (Human); 20423 (Mouse)
Alternate Names:
HHG1; HHG-1; HLP3; HPE3; MCOPCB5sonic hedgehog (Drosophila) homolog; Shh; SMMCIsonic hedgehog homolog (Drosophila); sonic hedgehog homolog; sonic hedgehog protein; Sonic Hedgehog; TPT; TPTPS
