Species Reactivity
Human, Mouse
Specificity
Detects mouse CCR6-transfected rat RBL cells but not the parent cell line or cells transfected with mouse CCR5. Detects human CCR6 in flow cytometry.
Source
Monoclonal Rat IgG 2A Clone # 140706
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
RBL-1 rat basophilic leukemia cell line transfected with mouse CCR6
Met1-Met367
Accession # O54689Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488
Applications
Recommended
ConcentrationSample
Flow Cytometry
5 µL/10 6 cells
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Flow Cytometry | Detection of CCR6 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes either (A) untreated or (B) treated with PMA, Calcium Ionomycin, LPS, and Recombinant Mouse IL‑23 (Catalog # ) overnight to induce Th17 activation were stained with Rat Anti-Mouse CD4 APC‑conjugated Monoclonal Antibody (Catalog # ) and either (A) Rat Anti-Mouse CCR6 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB590G) or (B) Rat IgG2A Alexa Fluor 488 Isotype Control (Catalog # ). View our protocol for . |
Flow Cytometry | Detection of CCR6 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD19 APC‑conjugated Monoclonal Antibody (Catalog # ) and either (A) Rat Anti-Human/Mouse CCR6 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB590G) or (B) Rat IgG2A Alexa Fluor 488 Isotype Control (Catalog # ). View our protocol for . |
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: CCR6
CCR6 is a seven transmembrane-spanning G protein-coupled receptor that binds the chemokine MIP-3 alpha and mediates the chemoattraction of B and T lymphocytes.
Entrez Gene IDs:
1235 (Human); 12458 (Mouse); 308163 (Rat)
Alternate Names:
BN-1; CC-CKR-6; CCR6; CCR-6; CD196 antigen; CD196; chemokine (C-C motif) receptor 6; CKR-L3G-protein coupled receptor 29; CKRL3LARC receptor; CMKBR6STRL22; DRY6; GPR29; GPR29C-C CKR-6; GPR-CY4; GPRCY4DRY-6; lymphocyte, 22; STRL22
