Mouse VEGF R1/Flt-1 Alexa Fluor 700 MAb (Clone 141522) 100 TESTS

• Species Reactivity

  Mouse

• Specificity

  Detects mouse VEGF R1/Flt‑1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human VEGF R1, recombinant mouse (rm) VEGF R2, or rmVEGF R3 is observed.

• Source

  Monoclonal Rat IgG    _2B Clone # 141522

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse VEGF R1/Flt‑1    
  Ser27-Glu759    
  Accession # P35969

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 700

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  5 µL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: VEGF R1/Flt-1

VEGF R1 is one of the five receptor tyrosine kinases (RTKs) (VEGF R1, KDR/Flk-1, Flt-4, Tie-1, and Tek/Tie-2) whose expression is almost exclusively restricted to the endothelial cells. Tie-1 and tek/tie-2 define a new class of RTKs containing two immunoglobulin-like domains, three EGF homology domains and three fibronectin type III domains in their extracellular regions. VEGF R1/Flt-1, VEGF R2/KDR/Flk-1, VEGF R3/Flt-4 are members of the class III subfamily of RTKs containing seven immunoglobulin-like repeats in their extracellular domains. All five RTKs are likely to play central roles in vasculogenesis and angiogenesis.

Full length mouse VEGF R1 mRNA encodes a 1333 amino acid (aa) residue precursor with a predicted 22 aa residue signal peptide. Mature VEGF R1 is composed of a 737 aa residue extracellular domain, a 22 aa residue transmembrane domain and a 552 aa residue cytoplasmic domain. As a result of alternative splicing of the mRNA, a cDNA encoding a truncated form of VEGF R1, lacking the seventh immunoglobulin-like domain, the transmembrane and intracellular domains, has been cloned. The recombinant soluble VEGF R1/Fc chimera binds VEGF and PlGF with high affinity and is a potent VEGF antagonist.

• References:

1. He, Y. et al. (1999) Molecular Endocrinology 13:537.

2. Ferrara, N. and T. Davis-Smyth (1997) Endocrine Reviews 8:4.

• Long Name:

  Vascular Endothelial Growth Factor Receptor 1

• Entrez Gene IDs:

  2321 (Human); 14254 (Mouse)

• Alternate Names:

  EC 2.7.10; EC 2.7.10.1; FLT; FLT1; Flt-1; Fms-like tyrosine kinase 1; fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascularpermeability factor receptor); FRT; Tyrosine-protein kinase FRT; Tyrosine-protein kinase receptor FLT; vascular endothelial growth factor receptor 1; Vascular permeability factor receptor; VEGF R1; VEGFR1; VEGFR-1