Species Reactivity
Human
Specificity
Detects human MICA in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with recombinant human MICB.
Source
Monoclonal Mouse IgG 2B Clone # 159227
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
BaF3 mouse pro-B cell line transfected with human MICA
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin
Applications
Recommended
ConcentrationSample
Flow Cytometry
10 µL/10 6 cells
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Flow Cytometry | Detection of MICA in K562 Human Cell Line by Flow Cytometry. K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human MICA PE‑conjugated Monoclonal Antibody (Catalog # FAB1300P, filled histogram) or isotype control antibody (Catalog # , open histogram). View our protocol for . |
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: MICA
MICA (MHC class I chain-related gene A) is a transmembrane glycoprotein that functions as a ligand for human NKG2D. A closely related protein, MICB, shares 85% amino acid identity with MICA. These proteins are distantly related to the MHC class I proteins. They possess three extracellular Ig-like domains, but they have no capacity to bind peptide or interact with beta 2-microglobulin. The genes encoding these proteins are found within the Major Histocompatibility Complex on human chromosome 6. The MICA locus is highly polymorphic with more than 50 recognized human alleles. MICA is absent from most cells but is frequently expressed in epithelial tumors and can be induced by bacterial and viral infections. MICA is a ligand for human NKG2D, an activating receptor expressed on NK cells, NKT cells, gamma δ T cells, and CD8+ alpha beta T cells. Recognition of MICA by NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. MICA recognition is involved in tumor surveillance, viral infections, and autoimmune diseases.
References:
Groh, V. et al. (2001) Nature Immunol. 2:255.
Stephens, H. (2001) Trends Immunol. 22:378.
Bauer, S. et al. (1999) Science 285:727.
Groh, V. et al. (2002) Nature 419:734.
Steinle, A. et al. (2001) Immunogenetics 53:279.
Pende, D. et al. (2002) Cancer Res. 62:6178.
NKG2D and its Ligands (2002) http://www.RnDSystems.com
Long Name:
MHC Class I-related Protein A
Entrez Gene IDs:
100507436 (Human)
Alternate Names:
FLJ60820; MGC111087; MICA; PERB11.1
