Species Reactivity
Human
Specificity
Detects human B7-H3 in direct ELISAs and Western blots. In Western blots, does not cross-react with recombinant human (rh) B7‑H1, rhB7‑H2, rhB7‑1, rhB7‑2, or recombinant mouse PD-L2.
Source
Monoclonal Mouse IgG 1 Clone # 185504
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human B7‑H3
Leu29-Pro245
Accession # NP_079516Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin
Applications
Recommended
ConcentrationSample
Flow Cytometry
10 µL/10 6 cells
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Flow Cytometry | Detection of B7‑H3 in Human Dendritic Cells by Flow Cytometry. Human monocyte-derived dendritic cells were stained with Mouse Anti-Human B7‑H3 PE‑conjugated Monoclonal Antibody (Catalog # FAB1027P, filled histogram) or isotype control antibody (Catalog # , open histogram). View our protocol for . |
Flow Cytometry | Detection of B7‑H3 in PC‑3 Human Cell Line by Flow Cytometry. PC‑3 human prostate cancer cell line was stained with Mouse Anti-Human B7‑H3 PE‑conjugated Monoclonal Antibody (Catalog # FAB1027P, filled histogram) or isotype control antibody (Catalog # , open histogram). View our protocol for . |
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: B7-H3
B7-H3 is a member of the B7 family of immune costimulatory receptors. Its expression is induced on dendritic cells and monocytes by inflammatory cytokines.
Long Name:
B7 Homolog 3
Entrez Gene IDs:
80381 (Human); 102657 (Mouse); 102131295 (Cynomolgus Monkey)
Alternate Names:
B7H3; B7-H3; B7H34Ig-B7-H3; B7-H3B7 homolog 3; CD276 antigen; CD276 molecule; CD276; Costimulatory molecule
