Human IL-6 Phycoerythrin MAb (Clone 1936) 100 TESTS

• Species Reactivity

  Human

• Specificity

  Detects human IL-6 in direct ELISAs. Does not cross-react with recombinant IL-6 from mouse, rat, or pig.

• Source

  Monoclonal Mouse IgG    _2B Clone # 1936

• Purification

  Protein A or G purified from ascites

• Immunogen

  E. coli-derived recombinant human IL‑6    
  Val30-Met212    
  Accession # P05231

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Phycoerythrin

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Intracellular Staining by Flow Cytometry

  10 µL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-6

Interleukin 6 (IL-6) is a pleiotropic alpha -helical cytokine that plays important roles in acute phase reactions, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6 activity is essential for the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. It is secreted by multiple cell types as a 22-28 kDa phosphorylated and variably glycosylated molecule (1-4). Mature human IL-6 is 183 amino acids (aa) in length and shares 41% aa sequence identity with mouse and rat IL-6 (5). Alternate splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties (6-9). Human IL-6 is equally active on mouse and rat cells (10). IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R, triggering IL-6 R association with gp130 and gp130 dimerization (11). gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM (12). Soluble forms of IL-6 R are generated by both alternate splicing and proteolytic cleavage (3). In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R elicit responses from gp130-expressing cells that lack cell surface IL-6 R (3). Trans‑signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R is predominantly restricted to hepatocytes, leukocytes, and lymphocytes (3). Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R but not from other cytokines that utilize gp130 as a coreceptor (4, 13).

• References:

1. Van Snick, J. (1990) Annu. Rev. Immunol. 8:253.

2. Hodge, D.R. et al. (2005) Eur. J. Cancer 41:2502.

3. Jones, S.A. (2005) J. Immunol. 175:3468.

4. Rose-John, S. et al. (2006) J. Leukoc. Biol. 80:227.

5. Hirano, T. et al. (1986) Nature 324:73.

6. Alberti, L. et al. (2005) Cancer Res. 65:2.

7. Kestler, D.P. et al. (1995) Blood 86:4559.

8. Kestler, D.P. et al. (1999) Am. J. Hematol. 61:169.

9. Bihl, M.P. et al. (2002) Am. J. Respir. Cell Mol. Biol. 27:48.

10. Chiu, C.P. et al. (1988) Proc. Natl. Acad. Sci. USA 85:7099.

11. Murakami, M. et al. (1993) Science 260:1808.

12. Muller-Newen, G. (2003) Sci. STKE 2003:PE40.

13. Mitsuyama, K. et al. (2006) Clin. Exp. Immunol. 143:125. 

• Long Name:

  Interleukin 6

• Entrez Gene IDs:

  3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 100034196 (Equine); 493687 (Feline); 100008733 (Rabbit)

• Alternate Names:

  B cell stimulatory factor-2; B-cell differentiation factor; BSF-2; BSF2CTL differentiation factor; CDF; HGFHSFIFNB2Hybridoma growth factor; IFN-beta-2; IL6; IL-6; IL-6B-cell stimulatory factor 2; Interferon beta-2; interleukin 6 (interferon, beta 2); interleukin BSF-2; interleukin-6; MGI-2A