Species Reactivity
Mouse
Specificity
Detects mouse IL‑17E/IL-25 in ELISAs. In ELISAs, this antibody does not cross-react with recombinant human IL‑17E, recombinant mouse IL‑17, IL‑17B, C, D, or F.
Source
Monoclonal Rat IgG 2B Clone # 207702
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse IL‑17E/IL-25
Val17-Ala169
Accession # NP_542767Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin
Applications
Recommended
ConcentrationSample
Intracellular Staining by Flow Cytometry
10 µL/10 6 cells
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry | Detection of IL‑17E/IL‑25 in Tramp‑C1 Mouse Cell Line by Flow Cytometry. Tramp‑C1 mouse prostate cancer cell line was stained with Rat Anti-Mouse IL‑17E/IL‑25 PE‑conjugated Monoclonal Antibody (Catalog # IC13991P, filled histogram) or isotype control antibody (Catalog # , open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # ) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # ). View our protocol for . |
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-17E/IL-25
The Interleukin 17 (IL-17) family proteins, comprising six members (IL-17, IL-17B through IL-17F), are secreted, structurally related proteins that share a conserved cysteine-knot fold near the C-terminus, but have considerable sequence divergence at the N-terminus. With the exception of IL-17B, which exists as a non-covalently linked dimer, all IL-17 family members are disulfide-linked dimers. IL-17 family proteins are pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions (1, 2).
Mouse IL-17E cDNA encodes a 169 amino acid residues (aa) precursor protein with a putative 16 aa signal peptide (5). Mature mouse IL-17E shares 76% and 91% amino sequence (aa) identity with mature human and rat IL-17E, respectively. Mouse IL-17E also shares from 24-32% sequence identity with the other mouse IL-17 family members. IL-17E expression was detected at very low levels by PCR in various peripheral tissues including brain, kidney, lung, prostate, testis, adrenal gland spinal cord, and trachea. IL-17E binds and activates IL-17 B Receptor (IL-17B R) (alternatively known as IL-17 Rh1, IL-17E R, and EVI27) (3, 4), which is expressed in kidney and liver, and at lower levels in brain, testis and other endocrine tissues. The expression of IL-17B R is up regulated under inflammatory conditions. Ligation of IL-17E to IL-17 RB induces activation of nuclear factor kappa-B and stimulates the production of the pro-inflamatory cytokine IL-8 (3). IL-17 has also been found to promote the expression of the prototypical Th2 genes (4, 5).
References:
Aggarwal, S. and A.L. Gurney (2002) J. Leukoc. Biol. 71:1.
Moseley, T.A. et al. (2003) Cytokine & Growth Factor Rev. 14:155.
Lee, J. et al. (2001) J. Biol. Chem. 276:1660.
Hurst, S.D. et al. (2002) J. Immunol. 169:443.
Pan, G. et al. (2001) J. Immunol. 167:6569.
Long Name:
Interleukin 17E
Entrez Gene IDs:
64806 (Human); 140806 (Mouse); 501996 (Rat)
Alternate Names:
IL17E; IL-17E; IL17Einterleukin-17E; IL25; IL-25; interleukin 17E; interleukin 25; Interleukin-17E; interleukin-25
