Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Meassure by its ability to enhance anti-CD3-induced IFN-gamma secretion of mouse CD3 + T cells. The ED 50 for this effect is 0.4-2 μg/mL.
Source
Mouse myeloma cell line, NS0-derived Ala20-Pro223, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
AnalysisAla20
Predicted Molecular Mass
23.7 kDa
SDS-PAGE
35-41 kDa, reducing conditions
7459-CD |
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Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | ||
Reconstitution Reconstitute at 100 μg/mL in PBS. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: CD84/SLAMF5
CD84, also known as Ly‑9B and SLAMF5, is a type I transmembrane protein in the SLAM subgroup of the CD2 family. SLAM family proteins regulate multiple aspects of immune system function (1). Mature mouse CD84 consists of a 200 amino acid (aa) extracellular domain (ECD) with two Ig‑like domains, a 21 aa transmembrane segment, and a 87 aa cytoplasmic domain with two immunoreceptor tyrosine‑based switch motifs (ITSMs) (2). Alternate splicing generates an additional isoform that is truncated following the first Ig‑like domain. CD84 exhibits homophilic binding which is mediated by the N‑terminal Ig‑like domain (3). Ligation induces tyrosine phosphorylation in the cytoplasmic ITSMs which then recruit the signaling adaptor molecules SAP (SLAM‑associated protein) and EAT‑2 (EWS/Fli1‑activated transcript 2) (4, 5). CD84 is expressed as a 60‑90 kDa molecule with extensive and cell type‑specific glycosylation (6‑8). It is widely expressed among hematopoietic cells including hematopoietic stem cells (8), myeloid cells ( e.g. macrophages, monocytes, dendritic cells, granulocytes, and mast cells) (4, 7‑10), platelets and megakaryocytes (3, 7, 8, 11), and lymphocytes. Within the T cell lineage, CD84 is expressed on thymocytes, CD4 -CD8 - cells, single positive CD4 or CD8 cells, NKT cells, and on mouse but not human NK cells (3, 4, 8, 9, 12). Within the B cell lineage, it is expressed on pro‑ and pre‑, mature, marginal zone, and memory B cells as well as plasma cells (4, 8, 13). CD84 signaling inhibits Fc epsilon RI-induced mast cell activation (10) but enhances platelet activation (11), LPS‑induced macrophage activation (8), T cell proliferation and IFN‑ gamma production (3, 5), and the interactions between T cells and B cells that are required for germinal center formation (14).
References:
Cannons, J.L. et al. (2011) Annu. Rev. Immunol. 29:665.
de la Fuente, M.A. et al. (1999) Immunogenetics 49:249.
Martin, M. et al. (2000) J. Immunol. 167:3668.
Tangye, S.G. et al. (2002) Eur. J. Immunol. 32:1640.
Tangye, S.G. et al. (2003) J. Immunol. 171:2485.
de la Fuente, M.A. et al. (1997) Blood. 90:2398.
Krause, S.W. et al. (2000) Biochem. J. 346:729.
Sintes, J. et al. (2010) J. Leukoc. Biol. 88:687.
Romero, X. et al. (2004) Tissue Antigens 64:132.
Alvarez-Errico, D. et al. (2011) J. Immunol. 187:5577.
Nanda, N. et al. (2005) Blood 106:3028.
Wang, N. et al. (2010) J. Immunol. 185:5683.
De Salort, J. et al. (2011) Immunol. Lett. 134:129.
Cannons, J.L. et al. (2010) Immunity 32:253.
Long Name:
SLAM Family Member 5
Entrez Gene IDs:
8832 (Human)
Alternate Names:
CD84 antigen (leukocyte antigen); CD84 antigen; CD84 molecule; CD84; Cell surface antigen MAX.3; DKFZp781E2378; hCD84; hly9-beta; leucocyte differentiation antigen CD84; leukocyte antigen CD84; Leukocyte differentiation antigen CD84; Ly-9B; mCD84; Signaling lymphocytic activation molecule 5; SLAM family member 5; SLAMF5; SLAMF5LY9B
