Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce cell death using Mv1Lu mink lung epithelial cells. The ED 50 for this effect is 0.3-1.5 μg/mL.
Source
Mouse myeloma cell line, NS0-derived
Mouse RELT/TNFRSF19L
(Met1-Gln169)
Accession # Q8BX43IEGRMDP Mouse IgG2A
(Glu98-Lys330)N-terminus C-terminus Accession #
N-terminal Sequence
AnalysisThr32
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
41.6 kDa (monomer)
SDS-PAGE
40-55 kDa, reducing condtions
7465-RT |
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Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | ||
Reconstitution Reconstitute at 500 μg/mL in PBS. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: RELT/TNFRSF19L
RELT (Receptor Expressed in Lymphoid Tissues) is a 46 kDa (predicted) type I transmembrane glycoprotein belonging to the tumor necrosis factor receptor superfamily and designated TNFRSF19‑like (TNFRSF19L) (1, 2). It is primarily expressed in hematopoietic tissues and peripheral blood leukocytes (2, 3). Mouse RELT cDNA encodes 436 amino acids (aa), including a 31 aa signal peptide, a 138 aa extracellular domain (ECD) containing a TNF receptor cysteine‑rich domain and a potential N‑linked glycosylation site, a 21 aa transmembrane domain, and a 246 aa cytoplasmic region that lacks a death domain (2). Within the ECD, mouse RELT shares 78%, 89%, 70%, 70% and 67% aa sequence homology with human, rat, canine, porcine and bovine RELT, respectively. Among TNFRSF members, the RELT extracellular domain is most closely related to that of TROY/TNFRSF19 and OX40 (2). Neither human nor mouse RELT bind any of the ~19 TNF superfamily ligands that have been tested (1). Two related transmembrane proteins, RELL1 and RELL2, have been identified in both human and mouse (3). RELL1 and 2 are coexpressed with and can interact with RELT, and are thought to modulate its signaling (3‑5). Intracellularly, RELT has been shown to bind the adaptor protein TRAF‑1 and activate the NF‑ kappa B pathway, the phospholipid scramblase PLSCR1, and the oxidative stress responsive protein OSR1 (2‑5). Another investigator notes association and signaling through SPAK, but not TRAF or NF kappa B (6). When overexpressed in HEK‑293 cells, RELT induces p38 and JNK signaling and activates apoptosis (4‑6).
References:
Bossen C. et al. (2006) J. Biol. Chem. 281:13964.
Sica, G. et al. (2001) Blood 97:2702.
Cusick, J.K. et al. (2006) Biochem. Biophys. Res. Commun. 340:535.
Cusick, J.K. et al. (2010) Cell. Immunol. 261:1.
Cusick, J.K. et al. (2012) Mol. Cell. Biochem. 362:55.
Polek T.C. et al. (2006) Biochem. Biophys. Res. Commun. 343:125.
Long Name:
Receptor Expressed in Lymphoid Tissues
Entrez Gene IDs:
84957 (Human); 320100 (Mouse)
Alternate Names:
Receptor expressed in lymphoid tissues; RELT tumor necrosis factor receptor; RELT; TNFRSF19L; TNFRSF19LFLJ14993; tumor necrosis factor receptor superfamily member 19L; tumor necrosis factor receptor superfamily, member 19-like
